Tanaka H, Morooka N, Haraikawa K, Ueno Y
Mutat Res. 1987 Feb;176(2):165-70. doi: 10.1016/0027-5107(87)90046-7.
Studies were undertaken to elucidate further the mechanism by which emodin, an anthraquinoid mycotoxin and constituent of rhubarb, was converted into a direct-acting mutagen to Salmonella typhimurium TA1537 by the hepatic microsomes and the reconstituted cytochrome P-450 system. Emodin was activated into a mutagenic principle(s) in the reconstituted cytochrome P-450 system, and its mutagenicity was significantly higher with the fraction II (P-448 type) than the fraction I (P-450 type) derived from the hepatic microsomes of PCB-induced rats. Thin-layer chromatographic analysis revealed that the purified cytochrome II-a (maximal CO-differential spectrum at 448.0 nm and high-spin form) activity converted emodin into 2-hydroxy-emodin, a direct-acting mutagen.
开展了多项研究，以进一步阐明大黄的蒽醌类霉菌毒素成分大黄素被肝微粒体和重组细胞色素P-450系统转化为鼠伤寒沙门氏菌TA1537的直接作用诱变剂的机制。大黄素在重组细胞色素P-450系统中被激活为诱变原，且与来自多氯联苯诱导大鼠肝微粒体的组分I（P-450型）相比，其在组分II（P-448型）中的诱变性显著更高。薄层色谱分析表明，纯化的细胞色素II-a（在448.0 nm处具有最大CO-差光谱且为高自旋形式）活性将大黄素转化为直接作用诱变剂2-羟基大黄素。
