在使用V79中国仓鼠细胞进行的次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）和姐妹染色单体交换（SCE）试验中，细菌诱变剂大黄素缺乏活性。

Lack of activity of the bacterial mutagen emodin in HGPRT and SCE assay with V79 Chinese hamster cells.

作者信息

Bruggeman I M, van der Hoeven J C

出版信息

Mutat Res. 1984 Nov-Dec;138(2-3):219-24. doi: 10.1016/0165-1218(84)90047-8.

Abstract

Genotoxicity of emodin was studied in the Salmonella/microsome assay, the sister-chromatid exchange (SCE) assay and the hypoxanthine-guanine-phosphoribosyltransferase (HGPRT) forward mutation assay with V79 Chinese hamster cells. In the Salmonella/microsome assay, emodin was found to be positive in TA97, TA100 and TA1537 in the presence of liver homogenate. In TA1537 a weak direct mutagenicity was also observed. In both mammalian test systems, no genotoxicity was found either with or without metabolic activation.

摘要

通过沙门氏菌/微粒体试验、姐妹染色单体交换（SCE）试验以及使用V79中国仓鼠细胞的次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）正向突变试验，对大黄素的遗传毒性进行了研究。在沙门氏菌/微粒体试验中，发现大黄素在有肝匀浆存在的情况下，对TA97、TA100和TA1537呈阳性反应。在TA1537中还观察到了微弱的直接致突变性。在这两种哺乳动物试验系统中，无论有无代谢活化，均未发现遗传毒性。