Stolk L M, Fruijtier A, Umans R
Pharm Weekbl Sci. 1986 Dec 12;8(6):286-8. doi: 10.1007/BF02280051.
Solutions of 0.6 mg/ml mitomycin C (Mutamycin) in NaCl solution 0.9% in plastic minibags for intravesical use were stored at -20 degrees C and -30 degrees C and thawed by microwave radiation. Both unbuffered solutions and solutions buffered to approximately pH 7.5 were investigated. Concentrations were measured with HPLC. During freezing and storage at -20 degrees C sometimes crystallization of mitomycin C occurred. However, both buffered and unbuffered solutions could be frozen at -30 degrees C during at least four weeks and subsequently thawed without loss of content. Unbuffered mitomycin C solutions could be refrozen at -30 degrees C for another four weeks. Also buffered and unbuffered solutions of another brand of mitomycin C (Mitomycin-C Kyowa) could be frozen at -30 degrees C during four weeks without significant deterioration.
用于膀胱内灌注的0.6毫克/毫升丝裂霉素C（丝裂霉素）溶液，置于含0.9%氯化钠溶液的塑料小袋中，分别储存在-20℃和-30℃，并通过微波辐射解冻。对未缓冲溶液和缓冲至pH约7.5的溶液均进行了研究。采用高效液相色谱法测定浓度。在-20℃冷冻和储存期间，丝裂霉素C有时会结晶。然而，缓冲溶液和未缓冲溶液在-30℃均可冷冻至少四周，随后解冻且含量无损失。未缓冲的丝裂霉素C溶液可在-30℃再次冷冻四周。另一个品牌的丝裂霉素C（协和发酵丝裂霉素）的缓冲溶液和未缓冲溶液在-30℃冷冻四周也不会显著变质。
