17个用于波纹蝠鲼（Mobula alfredi）的多态微卫星标记的开发与表征
Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi).
作者信息
Armstrong Amelia J, Dudgeon Christine L, Bustamante Carlos, Bennett Michael B, Ovenden Jennifer R
机构信息
School of Biomedical Science, The University of Queensland, Brisbane, Australia.
Facultad de Recursos Naturales Renovables, Universidad Arturo Prat, Iquique, Chile.
出版信息
BMC Res Notes. 2019 Apr 22;12(1):233. doi: 10.1186/s13104-019-4270-8.
Abstract
OBJECTIVE
Limited sample sizes are often a problem for species of conservation concern when using genetic tools to make population assessments. Lack of analytical power from small sample sizes can be compensated for by use of a large marker set. Here we report on development and characterization of 17 novel microsatellite markers for the reef manta ray (Mobula alfredi).
RESULTS
Loci were screened on 60 reef manta rays (M. alfredi) sampled from the east coast of Australia. The number of alleles per locus varied from 2 to 13 with observed heterozygosities ranging between 0.300 and 0.917. The development of these 17 additional markers increases the total number of microsatellite markers available for this species to 27.
摘要
目的
在使用遗传工具进行种群评估时，样本量有限常常是受保护物种面临的一个问题。小样本量导致的分析能力不足可通过使用大量标记集来弥补。在此，我们报告了为礁蝠鲼（Mobula alfredi）开发和鉴定的17个新型微卫星标记。
结果
在从澳大利亚东海岸采集的60只礁蝠鲼（M. alfredi）上对这些位点进行了筛选。每个位点的等位基因数量从2个到13个不等，观察到的杂合度在0.300至0.917之间。这17个额外标记的开发使该物种可用的微卫星标记总数增加到27个。
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