College of Life Science, Northwest Normal University, China.
College of Life Science, Northwest Normal University, China.
Plant Physiol Biochem. 2019 Jun;139:738-745. doi: 10.1016/j.plaphy.2019.04.020. Epub 2019 Apr 16.
Weisiensin B, a new ent-kaurene diterpenoid isolated from Isodon weisiensis (C. Y. Wu) H. Hara, exhibited phytotoxic effects on root growth and lateral root development in Arabidopsis thaliana seedlings. Primary root growth and lateral root formation in A. thaliana seedlings were significantly inhibited by 10-20 μM weisiensin B. Additionally, the role of weisiensin B in response to polar auxin transport in A. thaliana roots was investigated using a PIN promoter (PIN::GUS), a green fluorescent protein (GFP) fusion protein reporter (PINs::PINs:GFP), and DR5::GUS and DR5::GFP reporter genes. The results indicated that weisiensin B reduced the expression of PIN2, PIN3, PIN4, PIN7, and AUX1 genes and significantly decreased the abundance of PIN2-GFP, PIN3-GFP, PIN4-GFP, PIN7-GFP, and AUX1-GFP fusion proteins at their respective cellular locations, simultaneously causing auxin accumulation in the root apex. These results suggest that weisiensin B interferes with polar auxin transport in A. thaliana roots, resulting in auxin accumulation in the root meristematic cells and the inhibition of root growth and lateral root development.
韦斯新 B，一种从威氏香茶菜（C. Y. Wu）H. Hara 中分离得到的新的贝壳杉烯二萜类化合物，对拟南芥幼苗的根生长和侧根发育表现出植物毒性作用。10-20 μM 的韦斯新 B 显著抑制拟南芥幼苗的主根生长和侧根形成。此外，还使用 PIN 启动子（PIN::GUS）、绿色荧光蛋白（GFP）融合蛋白报告基因（PINs::PINs:GFP）以及 DR5::GUS 和 DR5::GFP 报告基因研究了韦斯新 B 在拟南芥根中响应极性生长素运输的作用。结果表明，韦斯新 B 降低了 PIN2、PIN3、PIN4、PIN7 和 AUX1 基因的表达，并显著减少了 PIN2-GFP、PIN3-GFP、PIN4-GFP、PIN7-GFP 和 AUX1-GFP 融合蛋白在各自细胞位置的丰度，同时导致生长素在根尖的积累。这些结果表明，韦斯新 B 干扰了拟南芥根中的极性生长素运输，导致生长素在根分生细胞中积累，并抑制根生长和侧根发育。
