Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, SI-1000, Ljubljana, Slovenia.
Protoplasma. 2022 Mar;259(2):343-355. doi: 10.1007/s00709-021-01668-4. Epub 2021 Jun 5.
Allelopathic compounds released by invasive alien plants can suppress the growth of plants in their vicinity. The aim of this study was to investigate changes in tissue and cell structure in roots of radish seedlings treated with 10% aqueous extracts of rhizomes from the invasive knotweeds Fallopia japonica and F. ×bohemica. After 7 days of growth without and with aqueous extracts from these rhizomes, the anatomical and ultrastructural changes in the radish seedling roots were analyzed with light and transmission electron microscopy, and hydrogen peroxide was localized with diaminobenzidine, to define oxidative stress. The roots of radish seedlings treated with the knotweed extracts were shorter and thicker, due to the shorter and wider shapes of their cortex cells, which were organized in more columns than the control roots. There were signs of cell damage and oxidative stress in the root cap cells, and to a lesser extent in the meristematic zone. As well as the irregularly shaped nuclei and plasma membrane detached from the cell wall, the most prominent ultrastructural effects in the root cap cells of these aqueous rhizome extracts were the ring-shaped form of the mitochondria and large endoplasmic reticulum bodies. Excessive vacuolization was seen for the cells of the root apical meristem.
外来入侵植物释放的化感化合物会抑制其周围植物的生长。本研究旨在研究萝卜幼苗根系组织和细胞结构在 10%入侵植物菰根和 F. × bohemica 水提物处理下的变化。在没有和有这些菰根水提取物的情况下生长 7 天后，用光学显微镜和透射电子显微镜分析萝卜幼苗根系的解剖和超微结构变化，并使用二氨基联苯胺定位过氧化氢，以定义氧化应激。用杂草提取物处理的萝卜幼苗的根更短、更粗，这是由于其皮层细胞的形状更短、更宽，比对照根组织的细胞排列成更多的柱状。根冠细胞有细胞损伤和氧化应激的迹象，在分生区则较少。除了细胞核形状不规则和质膜从细胞壁上脱离之外，根冠细胞中最显著的超微结构效应是线粒体呈环状和内质体较大。根尖分生区细胞出现过度液泡化。
