College of Life Sciences, Huzhou University, Huzhou 313000, China.
College of Life Sciences, Huzhou University, Huzhou 313000, China.
J Chromatogr A. 2019 Aug 16;1599:1-8. doi: 10.1016/j.chroma.2019.04.021. Epub 2019 Apr 12.
In the present work, mixed-mode chromatography adsorbents grafted with dextran of different molecular weight were prepared. Compared to non-grafted adsorbent (BA-6B), the morphology of dextran-grafted adsorbents (BA-6B-Ts) did not change significantly, while the water content (ω), porosity (P) and pore volume (V) decreased. Static adsorption, salt tolerance and dynamic adsorption behaviors of adsorbents BA-6B-Ts against BSA/IgG were studied. Compared to adsorbent BA-6B, adsorbents BA-6B-Ts had higher saturated adsorption capacity (Q) toward BSA/IgG, especially for BA-6B-T20, the Q values toward BSA and IgG increased by 32.8% and 39.5%, respectively. Adsorbents BA-6B-Ts showed enhanced salt tolerance toward BSA, and relatively weaker salt tolerance toward IgG. The relative adsorption capacity of BA-6B-T20 was 66.7% at 0.1 mol/L NaCl and 39.2% at 0.5 mol/L NaCl, higher than those of BA-6B. Moreover, for both BSA and IgG, the effective pore diffusivity (D) of BA-6B-Ts was higher than that of BA-6B. D values of BA-6B-T20 toward BSA and IgG increased by 77% and 139% compared to BA-6B, respectively. Dynamic binding capacities (DBCs) of BA-6B-Ts were also improved. DBC of BSA and IgG on BA-6B-T20 were 25.6% and 46.5% higher than those on BA-6B. The possible mechanisms for the above difference in adsorption behavior of dextran-grafted adsorbents toward BSA/IgG were discussed, providing some insights into protein adsorption and mass transport for dextran-grafted MMC resins.
在本工作中，制备了接枝不同分子量葡聚糖的混合模式色谱吸附剂。与非接枝吸附剂（BA-6B）相比，葡聚糖接枝吸附剂（BA-6B-Ts）的形态没有明显变化，而含水量（ω）、孔隙率（P）和孔体积（V）降低。研究了吸附剂 BA-6B-Ts 对 BSA/IgG 的静态吸附、耐盐性和动态吸附行为。与吸附剂 BA-6B 相比，吸附剂 BA-6B-Ts 对 BSA/IgG 的饱和吸附容量（Q）更高，特别是 BA-6B-T20 对 BSA 和 IgG 的 Q 值分别增加了 32.8%和 39.5%。吸附剂 BA-6B-Ts 对 BSA 表现出增强的耐盐性，而对 IgG 的耐盐性相对较弱。在 0.1mol/L NaCl 和 0.5mol/L NaCl 下，BA-6B-T20 的相对吸附容量分别为 66.7%和 39.2%，高于 BA-6B。此外，对于 BSA 和 IgG，BA-6B-Ts 的有效孔扩散系数（D）均高于 BA-6B。BA-6B-T20 对 BSA 和 IgG 的 D 值分别比 BA-6B 增加了 77%和 139%。BA-6B-Ts 的动态结合容量（DBC）也得到了提高。BA-6B-T20 对 BSA 和 IgG 的 DBC 分别比 BA-6B 高 25.6%和 46.5%。讨论了葡聚糖接枝吸附剂对 BSA/IgG 吸附行为差异的可能机制，为葡聚糖接枝 MMCR 中的蛋白质吸附和质量传递提供了一些见解。
