Kanoh T, Ohnaka T, Takamatsu T, Ohno T, Uchino H, Horiguchi Y
Scand J Haematol. 1986 Nov;37(5):443-7. doi: 10.1111/j.1600-0609.1986.tb02635.x.
Bone marrow aspirates from 10 patients with multiple myeloma (6 IgG myeloma, 3 IgA myeloma, 1 Bence Jones myeloma) were examined for nucleolus-associated localization of J chains. On light microscopy using the PAP method, almost all or a significant number of myeloma cells exhibited intranuclear spots stained with anti-J chains in 3 (IgG myeloma) out of the 8 cases positive for cytoplasmic J chains. In contrast, the nucleus of myeloma cells examined was constantly negative for anti-heavy and -light chains. Immunoelectron microscopically, J chain was identified as dicrete round electron-dense precipitates, corresponding to the whole nucleolus, and as sparsely distributed, small electron-dense deposits in the nucleus. In addition, some connections were found between nuclear and nucleolar electron-dense precipitates. Several possible explanations have been proposed to account for this localization of J chains.
对10例多发性骨髓瘤患者（6例IgG型骨髓瘤、3例IgA型骨髓瘤、1例本-周蛋白型骨髓瘤）的骨髓穿刺物进行检查，以观察J链的核仁相关定位。在使用PAP法的光学显微镜下，在8例胞质J链阳性的病例中，有3例（IgG型骨髓瘤）几乎所有或大量骨髓瘤细胞呈现出用抗J链染色的核内斑点。相比之下，所检查的骨髓瘤细胞核对抗重链和轻链始终呈阴性。免疫电子显微镜检查显示，J链被鉴定为对应于整个核仁的离散圆形电子致密沉淀物，以及在细胞核中稀疏分布的小电子致密沉积物。此外，在核内和核仁电子致密沉淀物之间发现了一些连接。已经提出了几种可能的解释来解释J链的这种定位。
