Yasuda N, Kanoh T, Uchino H
Clin Exp Immunol. 1980 Jun;40(3):573-80.
The presence of J chain in human immunocytes containing various immunoglobulin classes has already been reported and in this paper we investigated the presence of J chain in human myeloma cells using light and electron microscopy. Myeloma cells were positive for cytoplasmic J chain in thirteen cases: six of fifteen IgG myeloma, two of seven IgA myeloma, two of nine B-J myeloma and three of three primary macroglobulinaemia. None of the four with benign monoclonal gammopathy was positive for J chain. The ultrastructural localization of J chain in IgG myeloma cells was similar to that of gamma chain, i.e. around the nuclear membrane, in the endoplasmic reticulum (ER) and in the Golgi apparatus. The latter finding is compatible with the presence of J chain in the urine of the same patient. We examined the urine from nine patients with J chain-positive cells and secretion of J chain was confirmed in three patients: two with IgG myeloma and one with B-J myeloma. In noe of twelve in whom the immunocytochemical findings were negative for J chain was there evidence of urinary J chain.
已有报道称在含有各种免疫球蛋白类别的人类免疫细胞中存在J链，在本文中，我们使用光学显微镜和电子显微镜研究了人类骨髓瘤细胞中J链的存在情况。13例骨髓瘤细胞的细胞质J链呈阳性：15例IgG骨髓瘤中有6例，7例IgA骨髓瘤中有2例，9例B-J骨髓瘤中有2例，3例原发性巨球蛋白血症中有3例。4例良性单克隆丙种球蛋白病患者中J链均为阴性。IgG骨髓瘤细胞中J链的超微结构定位与γ链相似，即在核膜周围、内质网和高尔基体中。后一发现与同一患者尿液中存在J链相符。我们检查了9例J链阳性细胞患者的尿液，3例患者证实有J链分泌：2例IgG骨髓瘤患者和1例B-J骨髓瘤患者。12例免疫细胞化学检查J链呈阴性的患者中，均未发现尿J链的证据。
