Filyushin M A, Kochieva E Z, Shchennikova A V, Beletsky A V, Mardanov A V, Ravin N V, Skryabin K G
Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia.
Faculty of Biotechnology, Moscow State University, 119234, Moscow, Russia.
Dokl Biochem Biophys. 2019 May;484(1):29-32. doi: 10.1134/S1607672919010083. Epub 2019 Apr 22.
Fifteen chitinases of classes I-V were identified in the transcriptomes of pitchers and adult leaves of the carnivorous plant Nepenthes sp. Ten of these chitinases were identified for the first time, including the chitinases of classes II and V. The expression levels of all found chitinase genes in leaves and at three stages of pitcher development were determined. The maximum level of transcriptional activity in an open pitcher was observed for the genes encoding chitinase NChi4 (class II) and its isoforms. The expression levels of these genes significantly increased as the pitcher developed. In addition, for the first time, transcription of the genes encoding chitinases of all five classes was detected in the leaves of this plant.
在食肉植物猪笼草属物种的捕虫笼和成年叶片转录组中鉴定出了15种I-V类几丁质酶。其中10种几丁质酶是首次鉴定出来的，包括II类和V类几丁质酶。测定了所有发现的几丁质酶基因在叶片和捕虫笼发育三个阶段的表达水平。编码几丁质酶NChi4（II类）及其同工型的基因在开放的捕虫笼中观察到最大转录活性水平。随着捕虫笼的发育，这些基因的表达水平显著增加。此外，首次在该植物的叶片中检测到所有五类几丁质酶编码基因的转录。
