Khoroshko V A, Pokholkova G V, Zykova T Yu, Osadchiy I S, Zhimulev I F
Institute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia.
Institute of Gene Biology, Russian Academy of Sciences, 119334, Moscow, Russia.
Dokl Biochem Biophys. 2019 May;484(1):55-58. doi: 10.1134/S1607672919010137. Epub 2019 Apr 22.
The molecular and chromosomal localization of the dunce gene was studied. This gene (167.3 kb) consists almost entirely of introns, in which a cluster of seven short tissue-specific genes is located. On the basis of the results of FISH analysis of the gene fragments, we established that the dunce gene is located within nine chromosomal structures (four bands and five interbands), which contradicts the common idea that genes are located in only one structure (band or interband) or at the boundary of these structures. Our results are quite unexpected and original and greatly expand the current understanding of the genetic organization of interphase chromosomes.
对“笨蛋”基因的分子和染色体定位进行了研究。该基因（167.3 kb）几乎完全由内含子组成，其中定位有一组七个短的组织特异性基因。基于对基因片段的荧光原位杂交（FISH）分析结果，我们确定“笨蛋”基因位于九个染色体结构（四个带和五个带间区）内，这与基因仅位于一种结构（带或带间区）或这些结构的边界这一普遍观点相矛盾。我们的结果非常出人意料且具有原创性，极大地扩展了当前对间期染色体遗传组织的认识。
