Schmidt R, Moenke-Blankenburg L
Acta Histochem. 1986;80(2):205-13. doi: 10.1016/S0065-1281(86)80065-4.
Chemical analysis supported to a greater and greater degree by physical principles and measurement techniques offers some advantages for application in biomedical research and is able to complete histochemical procedures in elemental analysis. The described methods, such as electron-probe X-ray microanalysis, Auger electron spectrometry, cathodoluminescence analysis, electron diffraction, secondary ion mass spectrometry, electron spectrometry for chemical analysis, laser microprobes based on emission spectrometry, mass spectrometry and luminescence spectrometry yield qualitative and quantitative information about the inorganic (and organic) constituents and their distributions of subcellular microvolumes of biological tissues. Information content, figure of merit, applications, and limitations of these methods, and the special preparation techniques of biological materials are discussed.
化学分析在物理原理和测量技术的大力支持下，在生物医学研究应用中具有一些优势，并且能够完成元素分析中的组织化学程序。所描述的方法，如电子探针X射线微分析、俄歇电子能谱、阴极发光分析、电子衍射、二次离子质谱、化学分析电子能谱、基于发射光谱的激光微探针、质谱和发光光谱，可提供有关生物组织亚细胞微体积中无机（和有机）成分及其分布的定性和定量信息。讨论了这些方法的信息内容、品质因数、应用和局限性，以及生物材料的特殊制备技术。
