de Bruijn W C
Scan Electron Microsc. 1985(Pt 2):697-713.
It was investigated how to extract both morphometrical and X-ray elemental information from scanning electron microscopical (SEM) or scanning transmission electron microscopical (STEM)-images and how to integrate these two information streams either on line or off-line after storage. Cytochemical reaction products in cell organelles in ultrathin sections are the biological structures of interest. In such organelles four different situations can be met: morphologically the structures are homomorph or heteromorph; chemically the elements are distributed either homogeneously or heterogeneously. A new program has been proposed and described, which permits determination of both the area and the mean net-intensity value of chemical elements, inhomogeneously distributed over heteromorph organelles. The value of this integration method is demonstrated by three examples of increasing complexity, starting with two elements which are more or less homogeneously distributed over one lysosome, the establishing of a platinum discontinuity in an acidophilic granule and finally the localization of two chemical elements inhomogeneously distributed over a rather heteromorph phagolysosome. In two examples Chelex ion exchange beads, maximally loaded with the element also present in the structure of interest, are co-embedded with the tissue as internal standards. In such cases the absolute elemental concentration in the structures analysed can be established. The presence of such cross-sectioned beads in the ultrathin sections is also used: to demonstrate their function as models to select the proper conditions for the digital-controlled raster analysis of the unknown cell- or tissue structures, to prove the value of this method.
研究了如何从扫描电子显微镜（SEM）或扫描透射电子显微镜（STEM）图像中提取形态测量和X射线元素信息，以及如何在存储后在线或离线整合这两种信息流。超薄切片中细胞器的细胞化学反应产物是感兴趣的生物结构。在这样的细胞器中，可以遇到四种不同的情况：在形态上，结构是同形或异形的；在化学上，元素分布是均匀或不均匀的。已经提出并描述了一个新程序，该程序允许确定不均匀分布在异形细胞器上的化学元素的面积和平均净强度值。通过三个复杂度不断增加的例子展示了这种整合方法的价值，从两种元素或多或少均匀分布在一个溶酶体上开始，到在嗜酸性颗粒中建立铂的不连续性，最后到两种化学元素不均匀分布在一个相当异形的吞噬溶酶体上的定位。在两个例子中，用最大程度负载有也存在于感兴趣结构中的元素的螯合离子交换珠与组织共包埋作为内标。在这种情况下，可以确定分析结构中的绝对元素浓度。超薄切片中这种横截面珠的存在也被用于：证明它们作为模型的功能，以选择对未知细胞或组织结构进行数字控制光栅分析的合适条件，证明这种方法的价值。
