Dias de Castro Luciana L, Abrahão Carolina L H, Buzatti Andreia, Molento Marcelo B, Bastianetto Eduardo, Rodrigues Daniel S, Lopes Luciano B, Silva Marcos Xavier, de Freitas Mariana Green, Conde Mario Henrique, Borges Fernando de Almeida
Laboratory of Parasitic Diseases, Federal University of Parana, UFPR, R. dos Funcionários, 1540, Cabral, Curitiba, PR CEP 80.035-050, Brazil.
Laboratory of Parasitic Diseases, Federal University of Parana, UFPR, R. dos Funcionários, 1540, Cabral, Curitiba, PR CEP 80.035-050, Brazil.
Vet Parasitol Reg Stud Reports. 2017 Dec;10:132-135. doi: 10.1016/j.vprsr.2017.10.003. Epub 2017 Oct 10.
The aim of this study was to compare two fecal egg count (FEC) techniques; McMaster (McM) and Mini-FLOTAC (mF), for the detection of cattle and horse gastrointestinal nematode eggs, in different locations. Experiment 1: feces were collected from 16 cattle and FEC was performed individually, using mF with the sensitivity of 5 eggs per gram of feces (EPG) and McM with a sensitivity of 50 EPG at Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG and the Laboratory of Parasitic Diseases of the University of Parana - LDP/UFPR. Experiment 2: Fecal samples from 30 horses were analyzed with mF (sensitivity of 5 EPG) and McM (sensitivity of 25 EPG) at the University of Mato Grosso do Sul - UFMS and LPD/UFPR. Experiment 3: feces were collected from 14 foals and FEC was performed using mF (sensitivity of 5 EPG); and McM (sensitivity of 25 EPG) only at the LPD/UFPR. For cattle, the average FEC of mF was 962 at LPD; and 1248 at EPAMIG; for McM it was 1393 at LPD and 1563 at EPAMIG. For horses, the FEC average using the mF was 650 at LPD and 469 at UFMS; and for McM it was 677 at LPD and 554 at UFMS. For foals, the average FEC for mF was 404 and 436 for McM. In all experiments, the standard deviation and the coefficient of variation values were significantly lower for mF. Therefore, it is recommended the use of the Mini-FLOTAC technique, which is a method with less variability and higher accuracy, particularly for animals with low FEC.
本研究的目的是比较两种粪便虫卵计数（FEC）技术；麦克马斯特法（McM）和迷你FOLOTAC法（mF），用于检测不同地点牛和马的胃肠道线虫卵。实验1：从16头牛采集粪便，并分别使用粪便敏感度为每克5个虫卵（EPG）的mF和粪便敏感度为50 EPG的McM，在米纳斯吉拉斯州农业研究公司（EPAMIG）和巴拉那大学寄生虫病实验室（LDP/UFPR）进行FEC检测。实验2：在南马托格罗索州大学（UFMS）和LPD/UFPR，使用敏感度为5 EPG的mF和敏感度为25 EPG的McM对30匹马的粪便样本进行分析。实验3：从14匹小马驹采集粪便，并仅在LPD/UFPR使用敏感度为5 EPG的mF和敏感度为25 EPG的McM进行FEC检测。对于牛，LPD处mF的平均FEC为962；EPAMIG处为1248；McM在LPD处为1393，在EPAMIG处为1563。对于马，LPD处使用mF的FEC平均值为650，UFMS处为469；McM在LPD处为677，UFMS处为554。对于小马驹，mF的平均FEC为404，McM为436。在所有实验中，mF的标准差和变异系数值显著更低。因此，建议使用迷你FOLOTAC技术，该方法变异性较小且准确性较高，特别是对于FEC较低的动物。
