Ndom Mallé, Yanagida Tetsuya, Diop Gora, Quilichini Yann, Ba Aïssatou, Sako Yasuhito, Nakao Minoru, Marchand Bernard, Dieye Alioune, Ba Cheikh Tidiane, Ito Akira
Université Cheikh Anta Diop de Dakar, Laboratoire de Biologie Évolutive, d'Écologie et Gestion des Écosystèmes, Faculté des Sciences et Techniques, BP 5005 Dakar, Senegal.
Laboratory of Veterinary Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8515, Japan.
Vet Parasitol Reg Stud Reports. 2018 Jan;11:27-31. doi: 10.1016/j.vprsr.2017.11.008. Epub 2017 Nov 21.
Genetic and morphological diversity of Thysaniezia tapeworms from cattle and sheep in Senegal was investigated using light and scanning microscopic observations and molecular analysis based on mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear small subunit ribosomal RNA gene (SSU rDNA). A total of 52 adult tapeworms were collected from sheep and cattle. Although the tapeworms of the two hosts were morphologically very close, phylogenetic analysis based on cox1 and SSU rDNA gene sequences showed that they were divided into two clades corresponding each to a host. The maximum pairwise divergence between the clades were 12.1% in cox1 and 2.9% in SSU rDNA, indicating they are distinct species. The tapeworms collected from sheep were morphologically identified as Thysaniezia ovilla, a cosmopolitan species in domestic ruminants. Detailed morphological observations revealed a consistent difference between the tapeworms obtained from sheep and those from cattle. The latter were identified as Thysaniezia connochaeti. The present study highlights presence of two species of Thysaniezia among domestic ruminants in Senegal: T. ovilla specific to sheep and T. connochaeti specific to cattle. Our work is the first report of T. connochaeti from domestic animals.
利用光学显微镜和扫描显微镜观察以及基于线粒体细胞色素c氧化酶亚基1（cox1）和核糖体小亚基RNA基因（SSU rDNA）的分子分析，对塞内加尔牛和羊体内的膜壳绦虫的遗传和形态多样性进行了研究。总共从绵羊和牛身上采集了52条成虫绦虫。虽然这两种宿主的绦虫在形态上非常相似，但基于cox1和SSU rDNA基因序列的系统发育分析表明，它们被分为两个进化枝，分别对应于一种宿主。进化枝之间的最大成对差异在cox1中为12.1%，在SSU rDNA中为2.9%，表明它们是不同的物种。从绵羊身上采集的绦虫在形态上被鉴定为奥氏膜壳绦虫，这是一种在 domestic ruminants中广泛分布的物种。详细的形态学观察揭示了从绵羊身上获得的绦虫与从牛身上获得的绦虫之间存在一致的差异。后者被鉴定为康氏膜壳绦虫。本研究强调了塞内加尔家养反刍动物中存在两种膜壳绦虫：绵羊特有的奥氏膜壳绦虫和牛特有的康氏膜壳绦虫。我们的工作是关于康氏膜壳绦虫在家畜中出现的首次报道。 （注：原文中“domestic ruminants”未准确翻译，可理解为“家养反刍动物” ）
