Diop Gora, Yanagida Tetsuya, Hailemariam Zerihun, Menkir Sissay, Nakao Minoru, Sako Yasuhito, Ba Cheikh Tidiane, Ito Akira
Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal.
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510 Japan.
Parasitol Int. 2015 Oct;64(5):256-60. doi: 10.1016/j.parint.2015.02.008. Epub 2015 Mar 6.
Genetic diversity of Moniezia spp. from domestic ruminants in Senegal and Ethiopia was investigated based on the nucleotide sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear small subunit ribosomal RNA gene (SSU rDNA). A total of 64 adult tapeworms were collected from sheep, goat and cattle, and the tapeworms from cattle were all morphologically identified as Moniezia benedeni. On the other hand, the tapeworms obtained from sheep and goat were identified as Moniezia expansa or could not be identified because of the lack of diagnostic morphologic character, i.e. interproglottidal glands (IPGs). Phylogenetic analysis based on cox1 gene sequences revealed that the worms from sheep/goat and cattle formed distinct clades, and three mitochondrial lineages were confirmed within the sheep/goat tapeworms. The maximum pairwise divergences among the three mitochondrial linages were about 3% in cox1 and 0.1% in SSU rDNA, while that between the worms from sheep/goat and cattle reached 13% in cox1 and 2.7% in SSU rDNA. All of the three mitochondrial lineages contained tapeworms morphologically identified as M. expansa, and the tapeworms without IPGs were confirmed in one of the three lineages, indicating the tapeworms without IPGs were also M. expansa.
基于线粒体细胞色素c氧化酶亚基1（cox1）和核糖体小亚基RNA基因（SSU rDNA）的核苷酸序列，对来自塞内加尔和埃塞俄比亚家养反刍动物的莫尼茨绦虫属的遗传多样性进行了研究。共从绵羊、山羊和牛身上采集了64条成年绦虫，从牛身上采集的绦虫在形态上均被鉴定为贝氏莫尼茨绦虫。另一方面，从绵羊和山羊身上获得的绦虫被鉴定为扩展莫尼茨绦虫，或者由于缺乏诊断性形态特征，即节间腺（IPG）而无法鉴定。基于cox1基因序列的系统发育分析表明，来自绵羊/山羊和牛的绦虫形成了不同的进化枝，并且在绵羊/山羊绦虫中确认了三个线粒体谱系。三个线粒体谱系之间的最大成对差异在cox1中约为3%，在SSU rDNA中约为0.1%，而绵羊/山羊和牛的绦虫之间的差异在cox1中达到13%，在SSU rDNA中达到2.7%。所有三个线粒体谱系都包含形态上被鉴定为扩展莫尼茨绦虫的绦虫，并且在三个谱系之一中确认了没有节间腺的绦虫，这表明没有节间腺的绦虫也是扩展莫尼茨绦虫。
