Utaaker Kjersti Selstad, Tysnes Kristoffer Relling, Krosness Marie Myklatun, Robertson Lucy J
Parasitology Lab, Department for Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Adamstuen Campus, PO Box 8146, Dep. 0033, Oslo, Norway.
Parasitology Lab, Department for Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Adamstuen Campus, PO Box 8146, Dep. 0033, Oslo, Norway.
Vet Parasitol Reg Stud Reports. 2018 Dec;14:176-180. doi: 10.1016/j.vprsr.2018.10.008. Epub 2018 Oct 28.
Chandigarh, a city in North-west India, has numerous parks and recreational areas where stray dogs roam freely and pet dogs are exercised. This allows for extensive human-dog interaction, which may pose a public health threat. The aim of this study was to determine the occurrence of gastrointestinal parasites of public health importance, and their seasonal variation, in canine faecal samples obtained from recreational parks in Chandigarh. A total of 212 samples were collected from 10 parks during the winter (January 2015; N = 107) and monsoon season (September 2015; N = 105), to assess the prevalence of intestinal zoonotic parasites and any seasonal variations. The samples were analysed for helminth eggs using McMaster counting chambers. Immunofluorescent antibody testing was used to analyse samples for Cryptosporidium oocysts and Giardia cysts. The Giardia-positive samples were genotyped by conventional multi-locus PCR to determine their assemblage and zoonotic potential. Among the 212 samples, strongyle-type eggs were found in 34 (16.0%), Toxocara spp. eggs were found in 6 (2.8%), taeniid eggs in 1 (0.5%), Cryptosporidium spp. oocysts in 4 (1.9%) and Giardia duodenalis cysts in 49 (23.1%). Trichuris eggs were not detected. The majority of the successfully amplified Giardia isolates belonged to canid-specific assemblages. The prevalence of Giardia cysts in faecal samples was significantly higher during winter than in the monsoon season, whereas helminth-egg prevalence unaffected by season. The prevalence of strongyle-type eggs and Giardia cysts in dog faeces was lower in more affluent areas of the city than those of less affluence. There was no significant difference in the intensity of infection between the seasons. The results indicate that faeces from dogs contaminating parks in Chandigarh do not usually contain parasite transmission stages that pose a significant risk to human health. However, the importance of minimising contamination of public parks with dog faeces is highlighted.
印度西北部城市昌迪加尔有众多公园和休闲区，流浪狗在那里自由游荡，宠物狗也在这些地方活动。这使得人与狗之间有大量互动，可能构成公共卫生威胁。本研究的目的是确定从昌迪加尔休闲公园采集的犬粪便样本中具有公共卫生重要性的胃肠道寄生虫的发生率及其季节性变化。在冬季（2015年1月；N = 107）和季风季节（2015年9月；N = 105）从10个公园共采集了212份样本，以评估肠道人畜共患寄生虫的流行情况及任何季节性变化。使用麦克马斯特计数室分析样本中的蠕虫卵。采用免疫荧光抗体检测分析样本中的隐孢子虫卵囊和贾第虫囊肿。对贾第虫阳性样本进行常规多位点PCR基因分型，以确定其组合和人畜共患潜力。在212份样本中，发现圆线虫类虫卵的有34份（16.0%），弓蛔虫属虫卵6份（2.8%），带绦虫卵1份（0.5%），隐孢子虫属卵囊4份（1.9%），十二指肠贾第虫囊肿49份（23.1%）。未检测到鞭虫卵。大多数成功扩增的贾第虫分离株属于犬特异性组合。粪便样本中贾第虫囊肿的患病率在冬季显著高于季风季节，而蠕虫卵患病率不受季节影响。该市较富裕地区犬粪便中圆线虫类虫卵和贾第虫囊肿的患病率低于较不富裕地区。不同季节之间感染强度无显著差异。结果表明，昌迪加尔公园中被狗污染的粪便通常不含有对人类健康构成重大风险的寄生虫传播阶段。然而，强调了尽量减少公园被狗粪便污染的重要性。
