Obregón Dasiel, Corona Belkis G, de la Fuente José, Cabezas-Cruz Alejandro, Gonçalves Luiz Ricardo, Matos Carlos Antonio, Armas Yasmani, Hinojosa Yoandri, Alfonso Pastor, Oliveira Márcia C S, Machado Rosangela Z
Universidad Agraria de La Habana, Carretera Tapaste y Autopista Nacional, CP 32700, Apartado Postal 18-19, San José de Las Lajas, Mayabeque, Cuba; Centro Nacional de Sanidad Agropecuaria, Carretera de Jamaica y Autopista Nacional, CP 32700, Apartado Postal 10, San José de Las Lajas, Mayabeque, Cuba.
Centro Nacional de Sanidad Agropecuaria, Carretera de Jamaica y Autopista Nacional, CP 32700, Apartado Postal 10, San José de Las Lajas, Mayabeque, Cuba.
Vet Parasitol Reg Stud Reports. 2018 Aug;13:180-187. doi: 10.1016/j.vprsr.2018.06.007. Epub 2018 Jun 23.
Water buffalo (Bubalus bubalis) is a potential reservoir for Anaplasma marginale in livestock ecosystems of tropical countries. However, their participation in the epidemiological process of bovine anaplasmosis in endemic areas remains unclear. In the present study, the reservoir competence of water buffalo for A. marginale was explored by focusing on the analysis of rickettsemia levels in carrier animals, and the genetic characterization of A. marginale strains from cattle and buffalo. Eight groups of cattle and water buffaloes were randomly selected from cohabiting herds in four livestock ecosystems of Cuba, together with two control groups from unrelated cattle and buffalo herds. A total of 180 adult animals (88 water buffalo and 92 cattle) were sampled. Rickettsemia in carrier animals was determined by quantitative real-time PCR. The rickettsemia (parasitemia) levels in cattle were higher than in buffaloes, however the rickettsemia in buffalo may be enough to infect R. microplus ticks. The genetic diversity of A. marginale was assessed by strain characterization and phylogenetic analysis of 27 msp1α gene sequences. The results showed genetic similarity among strains from cattle and water buffalo, suggesting the occurrence of cross-species transmission.
在热带国家的家畜生态系统中，水牛（Bubalus bubalis）是边缘无浆体（Anaplasma marginale）的潜在宿主。然而，它们在地方病流行地区参与牛无浆体病流行病学过程的情况仍不清楚。在本研究中，通过关注带菌动物中立克次体血症水平的分析以及来自牛和水牛的边缘无浆体菌株的遗传特征，探讨了水牛对边缘无浆体的宿主能力。从古巴四个家畜生态系统中共同放牧的牛群和水牛群中随机选取八组牛和水牛，以及来自不相关牛群和水牛群的两个对照组。总共对180只成年动物（88头水牛和92头牛）进行了采样。通过定量实时PCR测定带菌动物中的立克次体血症。牛中的立克次体血症（寄生虫血症）水平高于水牛，但水牛中的立克次体血症可能足以感染微小牛蜱（R. microplus）。通过对27个msp1α基因序列进行菌株鉴定和系统发育分析，评估了边缘无浆体的遗传多样性。结果显示来自牛和水牛的菌株之间存在遗传相似性，表明发生了跨物种传播。
