University of Bordeaux, CNRS UMR 5295, I2M, F-33400, Talence, France.
University of Bordeaux, CNRS UMR 5248, Bordeaux INP, CBMN, F-33600, Pessac, France.
Sci Rep. 2019 Apr 23;9(1):6409. doi: 10.1038/s41598-019-42718-5.
Cell morphological analysis has long been used in cell biology and physiology for abnormality identification, early cancer detection, and dynamic change analysis under specific environmental stresses. This work reports on the remote mapping of cell 3D morphology with an in-plane resolution limited by optics and an out-of-plane accuracy down to a tenth of the optical wavelength. For this, GHz coherent acoustic phonons and their resonance harmonics were tracked by means of an ultrafast opto-acoustic technique. After illustrating the measurement accuracy with cell-mimetic polymer films we map the 3D morphology of an entire osteosarcoma cell. The resulting image complies with the image obtained by standard atomic force microscopy, and both reveal very close roughness mean values. In addition, while scanning macrophages and monocytes, we demonstrate an enhanced contrast of thickness mapping by taking advantage of the detection of high-frequency resonance harmonics. Illustrations are given with the remote quantitative imaging of the nucleus thickness gradient of migrating monocyte cells.
细胞形态分析在细胞生物学和生理学中一直用于异常识别、早期癌症检测以及特定环境应激下的动态变化分析。本工作报道了利用平面内分辨率受限于光学的细胞 3D 形态的远程映射，以及平面外精度达到光学波长十分之一的方法。为此，我们采用超快速光声技术跟踪了千兆赫兹相干声子及其共振谐波。在通过类似细胞的聚合物薄膜说明了测量精度之后，我们绘制了整个骨肉瘤细胞的 3D 形态。得到的图像与通过标准原子力显微镜获得的图像一致，并且两者都显示出非常接近的粗糙度平均值。此外，在扫描巨噬细胞和单核细胞时，我们通过利用高频共振谐波的检测来增强厚度映射的对比度。我们用迁移单核细胞的细胞核厚度梯度的远程定量成像进行了说明。
