Department of Physics, Ryerson University, Toronto, Ontario M5B2K3, Canada; Institute for Biomedical Engineering, Science and Technology (iBEST), a partnership between Ryerson University and St. Michael's Hospital, Toronto, Ontario, M5B1T8, Canada; Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, Ontario, M5B1T8, Canada.
Photoacoustics. 2016 Jan 18;4(1):36-42. doi: 10.1016/j.pacs.2016.01.001. eCollection 2016 Mar.
High resolution ultrasound and photoacoustic images of stained neutrophils, lymphocytes and monocytes from a blood smear were acquired using a combined acoustic/photoacoustic microscope. Photoacoustic images were created using a pulsed 532 nm laser that was coupled to a single mode fiber to produce output wavelengths from 532 nm to 620 nm via stimulated Raman scattering. The excitation wavelength was selected using optical filters and focused onto the sample using a 20× objective. A 1000 MHz transducer was co-aligned with the laser spot and used for ultrasound and photoacoustic images, enabling micrometer resolution with both modalities. The different cell types could be easily identified due to variations in contrast within the acoustic and photoacoustic images. This technique provides a new way of probing leukocyte structure with potential applications towards detecting cellular abnormalities and diseased cells at the single cell level.
使用组合式声学/光声显微镜获取血涂片上染色的中性粒细胞、淋巴细胞和单核细胞的高分辨率超声和光声图像。光声图像是通过将脉冲 532nm 激光耦合到单模光纤中产生的，通过受激拉曼散射产生 532nm 至 620nm 的输出波长。使用光学滤波器选择激发波长，并使用 20×物镜将其聚焦到样品上。1000MHz 换能器与激光点共轴，并用于超声和光声图像，两种模式都实现了微米级分辨率。由于声学和光声图像中的对比度变化，不同的细胞类型可以很容易地识别出来。该技术为探测白细胞结构提供了一种新方法，有可能应用于在单细胞水平上检测细胞异常和病变细胞。
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