萨夫登病毒-槟城 2B 和 3C 蛋白，但不是 L 蛋白，诱导 HEp-2 和 Vero 细胞凋亡。
The Saffold Virus-Penang 2B and 3C Proteins, but not the L Protein, Induce Apoptosis in HEp-2 and Vero Cells.
机构信息
Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore.
Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore, 117545, Singapore.
出版信息
Virol Sin. 2019 Jun;34(3):262-269. doi: 10.1007/s12250-019-00116-1. Epub 2019 Apr 23.
Our previous work has shown that Saffold virus (SAFV) induced several rodent and primate cell lines to undergo apoptosis (Xu et al. in Emerg Microb Infect 3:1-8, 2014), but the essential viral proteins of SAFV involved in apoptotic activity lack study. In this study, we individually transfected the viral proteins of SAFV into HEp-2 and Vero cells to assess their ability to induce apoptosis, and found that the 2B and 3C proteins are proapoptotic. Further investigation indicated the transmembrane domain of the 2B protein is essential for the apoptotic activity and tetramer formation of the 2B protein. Our research provides clues for the possible mechanisms of apoptosis induced by SAFV in different cell lines. It also opens up new directions to study viral proteins (the 2B, 3C protein), and sets the stage for future exploration of any possible link between SAFV, inclusive of its related uncultivable genotypes, and multiple sclerosis.
我们之前的工作表明，沙福病毒（SAFV）诱导几种啮齿动物和灵长类动物细胞系发生凋亡（Xu 等人，在《新发微生物感染》3：1-8，2014 年），但涉及凋亡活性的 SAFV 的必需病毒蛋白缺乏研究。在这项研究中，我们分别将 SAFV 的病毒蛋白转染到 HEp-2 和 Vero 细胞中，以评估它们诱导凋亡的能力，结果发现 2B 和 3C 蛋白具有促凋亡作用。进一步的研究表明，2B 蛋白的跨膜结构域对于 2B 蛋白的凋亡活性和四聚体形成是必需的。我们的研究为 SAFV 在不同细胞系中诱导凋亡的可能机制提供了线索。它也为研究病毒蛋白（2B、3C 蛋白）开辟了新的方向，并为未来探索 SAFV 之间的任何可能联系奠定了基础，包括其相关的不可培养基因型和多发性硬化症。
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