Faculty of Veterinary Science, Department of Preclinical and Applied Animal Science, Mahidol University, Nakhon Pathom, Thailand.
Center of Excellence in Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Adv Exp Med Biol. 2019;1123:151-164. doi: 10.1007/978-3-030-11096-3_9.
Epithelial damage in the salivary gland (SG) resulting in irreversible dry mouth can be commonly induced by gamma radiation therapy. This radiation depletes the SG stem/progenitor cell niche slowing healing and natural gland regeneration. Biologists have been focused in understanding the development and differentiation of epithelial stem and progenitor cell niches during SG organogenesis. These organogenesis studies gave insights into novel cell-based therapies to recreate the three-dimensional (3D) salivary gland (SG) organ, recapitulate the SG native physiology, and restore saliva secretion. Such therapeutical strategies apply techniques that assemble, in a 3D organotypic culture, progenitor and stem cell lines to develop SG organ-like organoids or mini-transplants. Future studies will employ a combination of organoids, decellularized matrices, and smart biomaterials to create viable and functional SG transplants to repair the site of SG injury and reestablish saliva production.
唾液腺 (SG) 的上皮损伤会导致不可逆转的口干，这通常是由伽马射线治疗引起的。这种辐射会耗尽 SG 干细胞/祖细胞龛，从而减缓愈合和自然腺体再生。生物学家一直专注于了解上皮干细胞和祖细胞龛在 SG 器官发生过程中的发育和分化。这些器官发生研究为基于细胞的新型治疗策略提供了见解，以重建三维 (3D) 唾液腺 (SG) 器官，再现 SG 的固有生理学并恢复唾液分泌。这些治疗策略应用了一些技术，将祖细胞和干细胞系组装在 3D 器官型培养物中，以开发 SG 类器官或迷你移植物。未来的研究将结合类器官、去细胞基质和智能生物材料，创建可行且功能正常的 SG 移植物，以修复 SG 损伤部位并重新建立唾液产生。
