一种用于人组织型纤溶酶原激活剂的活性位点滴定剂。
An active-site titrant for human tissue-type plasminogen activator.
作者信息
Smith R A
出版信息
Biochem J. 1986 Oct 15;239(2):477-9. doi: 10.1042/bj2390477.
Abstract
The reaction of recombinant tissue-type plasminogen activator with the inverse substrate 4-amidino-2-nitrophenyl 4'-anisate results in the rapid release of the chromogen 4-amidino-2-nitrophenol and the accumulation of the relatively stable 4-anisoyl-enzyme. Spectrophotometric monitoring of the reaction enables the operational molarity of the enzyme to be determined.
摘要
重组组织型纤溶酶原激活剂与反向底物4-脒基-2-硝基苯基4'-茴香酸酯反应，会迅速释放发色团4-脒基-2-硝基苯酚，并积累相对稳定的4-茴香酰化酶。通过分光光度法监测该反应能够测定酶的活性摩尔浓度。
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