位点特异性冠醚与DNA无碱基位点加合物的合成：8-氧代-7,8-二氢-2'-脱氧鸟苷和2'-脱氧胞苷。

Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites: 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and 2'-Deoxycytidine.

作者信息

An Na, Fleming Aaron M, Rosecrans Nicole C, Liao Yi, Burrows Cynthia J

机构信息

Department of Chemistry, University of Utah, Salt Lake City, UT, USA.

出版信息

Methods Mol Biol. 2019;1973:15-25. doi: 10.1007/978-1-4939-9216-4_2.

DOI:10.1007/978-1-4939-9216-4_2

PMID:31016693

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6482849/

Abstract

Formation of adducts to DNA is of great benefit to DNA sequencing and damage detection technology and to enzymology. Here we describe the synthesis and characterization procedures of 18-crown-6 adducts formed to abasic (AP) sites, 8-oxo-7,8-dihydro-2'-deoxyguanosine (OG), and 2'-deoxycytidine (C) residues in DNA oligodeoxynucleotides. These crown ether adducts were used as site-specific modifications to facilitate nanopore technology. The methods described can be readily expanded to attach other suitable primary amines of interest.

摘要

与DNA形成加合物对DNA测序、损伤检测技术以及酶学都非常有益。在此，我们描述了在DNA寡脱氧核苷酸中与无碱基（AP）位点、8-氧代-7,8-二氢-2'-脱氧鸟苷（OG）和2'-脱氧胞苷（C）残基形成的18-冠-6加合物的合成及表征程序。这些冠醚加合物用作位点特异性修饰以促进纳米孔技术。所描述的方法可以很容易地扩展到连接其他合适的目标伯胺。