调节α-溶血素离子通道中 DNA 无碱基位点加合物的电流特征。
Modulation of the current signatures of DNA abasic site adducts in the α-hemolysin ion channel.
机构信息
Department of Chemistry, University of Utah, Salt Lake City, USA.
出版信息
Chem Commun (Camb). 2012 Dec 4;48(93):11410-2. doi: 10.1039/c2cc36366f. Epub 2012 Oct 17.
Abstract
Electrical current signatures of DNA adducts were investigated during immobilization of strands inside the membrane-bound α-hemolysin ion channel. The current blockages produced by these adducts were found to depend on both size and shape, providing insights into the DNA-protein interactions and the size limitation of bulky adducts to be translocated.
摘要
在将 DNA 链固定在膜结合的α-溶血素离子通道内时，研究了 DNA 加合物的电流特征。这些加合物产生的电流阻断取决于大小和形状，为 DNA-蛋白质相互作用和大体积加合物的迁移大小限制提供了深入了解。
相似文献
1
Modulation of the current signatures of DNA abasic site adducts in the α-hemolysin ion channel.调节α-溶血素离子通道中 DNA 无碱基位点加合物的电流特征。
Chem Commun (Camb). 2012 Dec 4;48(93):11410-2. doi: 10.1039/c2cc36366f. Epub 2012 Oct 17.
2
Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel.利用离子通道以单核苷酸分辨率对单个DNA发夹分子进行快速区分。
Nat Biotechnol. 2001 Mar;19(3):248-52. doi: 10.1038/85696.
3
Nanopore detection of 8-oxo-7,8-dihydro-2'-deoxyguanosine in immobilized single-stranded DNA via adduct formation to the DNA damage site.通过与 DNA 损伤部位形成加合物，在固定化单链 DNA 中对 8-氧代-7,8-二氢-2'-脱氧鸟苷进行纳米孔检测。
J Am Chem Soc. 2010 Dec 29;132(51):17992-5. doi: 10.1021/ja109501x. Epub 2010 Dec 7.
4
Membrane Position Dependency of the pK and Conductivity of the Protein Ion Channel.蛋白质离子通道的pK值和电导率的膜位置依赖性。
J Membr Biol. 2018 Jun;251(3):393-404. doi: 10.1007/s00232-018-0013-3. Epub 2018 Jan 16.
5
6
Explicit channel conductance: can it be computed?明确的通道电导：它能被计算出来吗？
Biophys J. 2005 Jun;88(6):3742-3. doi: 10.1529/biophysj.105.060996. Epub 2005 Mar 11.
7
Planar microelectrode-cavity array for high-resolution and parallel electrical recording of membrane ionic currents.用于膜离子电流高分辨率并行电记录的平面微电极腔阵列
Lab Chip. 2008 Jun;8(6):938-44. doi: 10.1039/b800431e. Epub 2008 Apr 16.
8
Sensing proteins outside of the box.突破常规感知蛋白质。
Nat Biotechnol. 2000 Oct;18(10):1037. doi: 10.1038/80217.
9
Sequence-dependent gating of an ion channel by DNA hairpin molecules.DNA发夹分子对离子通道的序列依赖性门控作用。
Nucleic Acids Res. 2006;34(22):6425-37. doi: 10.1093/nar/gkl754. Epub 2006 Nov 27.
10
Aerolysin, a hemolysin from Aeromonas hydrophila, forms voltage-gated channels in planar lipid bilayers.气单胞菌溶素是嗜水气单胞菌产生的一种溶血素，可在平面脂质双分子层中形成电压门控通道。
J Membr Biol. 1990 Apr;115(1):71-81. doi: 10.1007/BF01869107.
引用本文的文献
1
Structure and Dynamics of dsDNA in Cell-like Environments.类细胞环境中双链DNA的结构与动力学
Entropy (Basel). 2022 Nov 1;24(11):1587. doi: 10.3390/e24111587.
2
Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites: 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and 2'-Deoxycytidine.位点特异性冠醚与DNA无碱基位点加合物的合成：8-氧代-7,8-二氢-2'-脱氧鸟苷和2'-脱氧胞苷。
Methods Mol Biol. 2019;1973:15-25. doi: 10.1007/978-1-4939-9216-4_2.
3
Characterization of Interstrand DNA-DNA Cross-Links Using the α-Hemolysin Protein Nanopore.使用α-溶血素蛋白纳米孔对链间DNA-DNA交联进行表征
ACS Nano. 2015 Dec 22;9(12):11812-9. doi: 10.1021/acsnano.5b03923. Epub 2015 Nov 18.
4
Identification of DNA lesions using a third base pair for amplification and nanopore sequencing.利用第三个碱基对进行扩增和纳米孔测序来鉴定DNA损伤。
Nat Commun. 2015 Nov 6;6:8807. doi: 10.1038/ncomms9807.
5
Detection of benzo[a]pyrene-guanine adducts in single-stranded DNA using the α-hemolysin nanopore.利用α-溶血素纳米孔检测单链DNA中的苯并[a]芘-鸟嘌呤加合物。
Nanotechnology. 2015 Feb 20;26(7):074002. doi: 10.1088/0957-4484/26/7/074002. Epub 2015 Jan 28.
6
The evolution of nanopore sequencing.纳米孔测序的发展历程。
Front Genet. 2015 Jan 7;5:449. doi: 10.3389/fgene.2014.00449. eCollection 2014.
7
Single-molecule detection of a guanine(C8) - thymine(N3) cross-link using ion channel recording.使用离子通道记录对鸟嘌呤（C8）-胸腺嘧啶（N3）交联进行单分子检测。
J Phys Org Chem. 2014 Apr 1;27(4):247-251. doi: 10.1002/poc.3240.
8
Programming nanopore ion flow for encoded multiplex microRNA detection.用于编码多重微小RNA检测的纳米孔离子流编程
ACS Nano. 2014 Apr 22;8(4):3444-50. doi: 10.1021/nn406339n. Epub 2014 Mar 26.
9
Electrical Current Signatures of DNA Base Modifications in Single Molecules Immobilized in the α-Hemolysin Ion Channel.固定于α-溶血素离子通道中的单分子DNA碱基修饰的电流特征
Isr J Chem. 2013 Jun 1;53(6-7):417-430. doi: 10.1002/ijch.201300022.
本文引用的文献
1
Crown ether-electrolyte interactions permit nanopore detection of individual DNA abasic sites in single molecules.冠醚-电解质相互作用允许在单个分子中单碱基错配的单个 DNA 碱基的纳米孔检测。
Proc Natl Acad Sci U S A. 2012 Jul 17;109(29):11504-9. doi: 10.1073/pnas.1201669109. Epub 2012 Jun 18.
2
Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase.利用突变型 MspA 纳米孔和 phi29 DNA 聚合酶实现单核苷酸分辨率下的 DNA 读取。
Nat Biotechnol. 2012 Mar 25;30(4):349-53. doi: 10.1038/nbt.2171.
3
Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision.在纳米孔中以 5Å 的精度实现 DNA 的自动正向和反向棘轮作用。
Nat Biotechnol. 2012 Feb 14;30(4):344-8. doi: 10.1038/nbt.2147.
4
Nucleobase recognition at alkaline pH and apparent pKa of single DNA bases immobilised within a biological nanopore.在碱性 pH 值下的碱基识别和固定在生物纳米孔内的单个 DNA 碱基的表观 pKa。
Chem Commun (Camb). 2012 Feb 1;48(10):1520-2. doi: 10.1039/c1cc16124e. Epub 2011 Nov 17.
5
Controlled translocation of individual DNA molecules through protein nanopores with engineered molecular brakes.利用工程化分子刹车控制单个 DNA 分子穿过蛋白质纳米孔的转运。
Nano Lett. 2011 Feb 9;11(2):746-50. doi: 10.1021/nl1038874. Epub 2011 Jan 11.
6
Nanopore detection of 8-oxo-7,8-dihydro-2'-deoxyguanosine in immobilized single-stranded DNA via adduct formation to the DNA damage site.通过与 DNA 损伤部位形成加合物，在固定化单链 DNA 中对 8-氧代-7,8-二氢-2'-脱氧鸟苷进行纳米孔检测。
J Am Chem Soc. 2010 Dec 29;132(51):17992-5. doi: 10.1021/ja109501x. Epub 2010 Dec 7.
7
Emergence of symmetry and chirality in crown ether complexes with alkali metal cations.冠醚配合物与碱金属阳离子的对称性和手性的出现。
J Phys Chem A. 2010 Jul 8;114(26):7048-54. doi: 10.1021/jp103389g.
8
Urea facilitates the translocation of single-stranded DNA and RNA through the alpha-hemolysin nanopore.尿素促进单链 DNA 和 RNA 通过α-溶血素纳米孔的易位。
Biophys J. 2010 May 19;98(9):1856-63. doi: 10.1016/j.bpj.2009.12.4333.
9
Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores.环糊精适配体与工程蛋白纳米孔相互作用的分子基础。
Proc Natl Acad Sci U S A. 2010 May 4;107(18):8165-70. doi: 10.1073/pnas.0914229107. Epub 2010 Apr 16.
10
Multiple base-recognition sites in a biological nanopore: two heads are better than one.生物纳米孔中的多个碱基识别位点：两个总比一个好。
Angew Chem Int Ed Engl. 2010;49(3):556-9. doi: 10.1002/anie.200905483.
Zotero 插件