1,25-二羟维生素D3对胞浆游离钙浓度的影响。

Influence of 1,25-hydroxyvitamin D3 on cytosolic free calcium concentrations.

作者信息

Bar S, Lidor C, Harell A, Edelstein S

出版信息

Biochem Biophys Res Commun. 1986 Dec 30;141(3):1236-41. doi: 10.1016/s0006-291x(86)80177-2.

Abstract

Using isolated cartilage cells from the epiphyseal growth plate of rachitic chicks and utilizing the fluorescence indicator quin2 for measurements of cytosolic free calcium, it has been possible to demonstrate that 1,25-dihydroxyvitamin D3 regulates the concentrations of cytosolic free calcium. The reduction in cytosolic free calcium is associated with inhibition of the activity of alkaline phosphatase. As several hours are required before an effect on cytosolic free calcium can be observed in the cells, it is suggested that the action is dependent on genomic interactions.

摘要

利用来自患佝偻病雏鸡骺生长板的分离软骨细胞，并使用荧光指示剂喹啉-2来测量细胞质游离钙，已经有可能证明1,25-二羟基维生素D3调节细胞质游离钙的浓度。细胞质游离钙的减少与碱性磷酸酶活性的抑制有关。由于在细胞中观察到对细胞质游离钙的影响之前需要几个小时，因此表明该作用依赖于基因组相互作用。