Kriajev L, Edelstein S
Biochemistry Department, Weizmann Institute of Science, Rehovot, Israel.
Biochem Biophys Res Commun. 1994 Aug 15;202(3):1204-8. doi: 10.1006/bbrc.1994.2058.
The effect of 24,25-dihydroxyvitamin D3 on some of the activities of 1,25-dihydroxyvitamin D3 was studied in cultures of cartilage cells obtained from the tibial growth plate of rachitic chicks. The activities that were tested are those associated with intracellular exchangeable calcium, alkaline-phosphatase activity and the incorporation of thymidine and proline. 24,25-dihydroxyvitamin D3 was shown in this system to antagonize the suppressive effect of 1,25-dihydroxyvitamin D3 on intracellular exchangeable calcium and on proline incorporation. A similar effect on alkaline phosphatase activity and thymidine incorporation was not observed.
在从佝偻病雏鸡胫骨生长板获取的软骨细胞培养物中，研究了24,25 - 二羟基维生素D3对1,25 - 二羟基维生素D3某些活性的影响。所测试的活性是那些与细胞内可交换钙、碱性磷酸酶活性以及胸苷和脯氨酸掺入相关的活性。在该系统中表明，24,25 - 二羟基维生素D3可拮抗1,25 - 二羟基维生素D3对细胞内可交换钙和脯氨酸掺入的抑制作用。未观察到对碱性磷酸酶活性和胸苷掺入有类似影响。
