Wan Jin'e, Yang Jian, Qiao Cuixia, Sun Xiaomei, Di Aiting, Zhang Lize, Wang Dandan, Zhao Gang
Department of Hyperbaric Oxygen, The Affiliated Hospital of Qingdao University, Qingdao, China.
Department of Oncology, Zouping Centre Hospital, Binzhou, China.
Yonsei Med J. 2019 May;60(5):414-422. doi: 10.3349/ymj.2019.60.5.414.
Colorectal cancer (CRC) is the third most common cancer in China and poses high morbidity and mortality. In recent years, increasing evidence has indicated that microRNAs played important functions in the occurrence and development of tumors. The purpose of this study was to identify the biological mechanisms of miR-362 in CRC.
Quantitative real-time PCR was carried out to assess the expression of miR-362 and SIX1. The Kaplan-Meier method was employed to evaluate the 5-year overall survival of CRC patients. The proliferative and invasive abilities of CRC cells were assessed by MTT and transwell assays.
miR-362 was significantly decreased in CRC tissues and cell lines, compared to the normal tissues and normal cells. A significant connection was confirmed between the overall survival of 53 CRC patients and low expression of miR-362. Downregulation of miR-362 inhibited the proliferation and invasion through binding to the 3'-UTR of SIX1 mRNA in CRC. Additionally, we discovered that SIX1 was a direct target gene of miR-362 and that the expression of miR-362 had a negative connection with SIX1 expression in CRC. SIX1 could reverse partial functions in the proliferation and invasion in CRC cells.
miR-362 may be a prognostic marker in CRC and suppress CRC cell proliferation and invasion in part through targeting the 3'-UTR of SIX1 mRNA. The newly identified miR-362/SIX1 axis provides insight into the progression of CRC.
结直肠癌（CRC）是中国第三大常见癌症，具有高发病率和死亡率。近年来，越来越多的证据表明，微小RNA在肿瘤的发生和发展中发挥着重要作用。本研究的目的是确定miR-362在结直肠癌中的生物学机制。
采用定量实时PCR评估miR-362和SIX1的表达。采用Kaplan-Meier法评估结直肠癌患者的5年总生存率。通过MTT和transwell实验评估结直肠癌细胞的增殖和侵袭能力。
与正常组织和正常细胞相比，miR-362在结直肠癌组织和细胞系中显著降低。53例结直肠癌患者的总生存率与miR-362低表达之间存在显著关联。miR-362的下调通过与结直肠癌中SIX1 mRNA的3'-UTR结合来抑制增殖和侵袭。此外，我们发现SIX1是miR-362的直接靶基因，并且miR-362的表达与结直肠癌中SIX1的表达呈负相关。SIX1可以逆转结直肠癌细胞增殖和侵袭中的部分功能。
miR-362可能是结直肠癌的一个预后标志物，并部分通过靶向SIX1 mRNA的3'-UTR来抑制结直肠癌细胞的增殖和侵袭。新发现的miR-362/SIX1轴为结直肠癌的进展提供了新的见解。
