miR-362-3p 通过靶向 SERBP1 在卵巢癌中发挥肿瘤抑制作用。
miR-362-3p acts as a tumor suppressor by targeting SERBP1 in ovarian cancer.
机构信息
Department of Obstetrics and Gynecology, Shanghai Songjiang District Central Hospital, 748, Zhongshan Middle Road, Songjiang District, Shanghai, China.
Department of Obstetrics and Gynecology, the First People's Hospital of Chenzhou, Southern Medical University, Chenzhou, China.
出版信息
J Ovarian Res. 2021 Feb 1;14(1):23. doi: 10.1186/s13048-020-00760-2.
BACKGROUND
Ovarian cancer is the leading lethal gynecological cancer and is generally diagnosed during late-stage presentation. In addition, patients with ovarian cancer still face a low 5-year survival rate. Thus, innovative molecular targeting agents are required to overcome this disease. The present study aimed to explore the function of miR-362-3p and the underlying molecular mechanisms influencing ovarian cancer progression.
METHODS
The expression levels of miR-362-3p were determined using qRT-PCR. Gain-of-function and loss-of-function methods were used to detect the effects of miR-362-3p on cell proliferation, cell migration, and tumor metastasis in ovarian cancer. A luciferase reporter assay was performed to confirm the potential target of miR-362-3p, and a rescue experiment was employed to verify the effect of miR-362-3p on ovarian cancer by regulating its target gene.
RESULTS
miR-362-3p was significantly downregulated in ovarian cancer tissues and cell lines. In vitro, our data showed that miR-362-3p suppressed cell proliferation and migration. In vivo, miR-362-3p inhibited ovarian cancer growth and metastasis. Mechanistically, SERBP1 was identified as a direct target and functional effector of miR-362-3p in ovarian cancer. Moreover, SERBP1 overexpression rescued the biological function of miR-362-3p.
CONCLUSIONS
Our data reveal that miR-362-3p has an inhibitory effect on ovarian cancer. miR-362-3p inhibits the development and progression of ovarian cancer by directly binding its target gene SERBP1.
背景
卵巢癌是导致女性死亡的主要妇科癌症，通常在晚期才被诊断出来。此外，卵巢癌患者的 5 年生存率仍然很低。因此，需要创新的分子靶向药物来攻克这种疾病。本研究旨在探讨 miR-362-3p 的功能及其影响卵巢癌进展的潜在分子机制。
方法
采用 qRT-PCR 检测 miR-362-3p 的表达水平。利用 gain-of-function 和 loss-of-function 方法检测 miR-362-3p 对卵巢癌细胞增殖、细胞迁移和肿瘤转移的影响。通过荧光素酶报告实验证实 miR-362-3p 的潜在靶基因，并通过调控其靶基因来验证 miR-362-3p 对卵巢癌的作用。
结果
miR-362-3p 在卵巢癌组织和细胞系中表达显著下调。体外实验表明，miR-362-3p 抑制细胞增殖和迁移。体内实验表明，miR-362-3p 抑制卵巢癌生长和转移。机制研究表明，SERBP1 是 miR-362-3p 在卵巢癌中的直接靶基因和功能效应因子。此外，SERBP1 的过表达可挽救 miR-362-3p 的生物学功能。
结论
本研究数据表明，miR-362-3p 对卵巢癌具有抑制作用。miR-362-3p 通过直接结合其靶基因 SERBP1 抑制卵巢癌的发生和发展。
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