Paiva-Guimarães A G L, Freire K R L, Santos S F M, Almeida A F, Sousa A C B
Laboratório de Genética Molecular e Biotecnologia Vegetal, Programa de Pós-graduação em Biotecnologia, Departamento de Biologia Celular e Molecular, Centro de Biotecnologia - CBIOTEC, Universidade Federal da Paraíba - UFPB, Cidade Universitária, Castelo Branco, s/n, CEP 58051-900, João Pessoa, PB, Brasil.
Laboratório de Tecnologia Cervejeira, Departamento de Biologia Celular e Molecular, Centro de Biotecnologia - CBIOTEC, Universidade Federal da Paraíba - UFPB, Cidade Universitária, Castelo Branco, s/n, CEP 58051-900, João Pessoa, PB, Brasil.
Braz J Biol. 2020 Jan-Feb;80(1):133-141. doi: 10.1590/1519-6984.195711.
Beauveria bassiana is a promising fungus for the biological control of insect pests. The growing costs of conidia production have raised the need to ascertain the efficiency of some low cost substrates. The aim of this study was to analyze the potential use of different raw substrates without nutritional supplement for B. bassiana conidiogenesis. Growth and sporulation were evaluated using 30 g of substrate and 0.3 μL of a conidia suspension (1 x 106 conidia/mL). After 10 days of incubation (70 ± 10% humidity and temperature (T) = 29 ± 1 °C), rice (2.00 x 106 conidia/g substrate), algaroba (2.36 x 106 conidia/g), malt A (1.22 x 106 conidia/g) and malt B (1.75 x 106 conidia/g) showed the highest levels of conidia production. The resulting conidia showed insecticidal activity higher than 80% on coconut termites. These new raw substrates may represent viable alternatives for the production of entomopathogenic fungi for use in the biological control of various insect pests.
球孢白僵菌是一种用于生物防治害虫的有前景的真菌。分生孢子生产成本的不断增加，使得确定一些低成本基质的效率变得很有必要。本研究的目的是分析不同无营养补充的原始基质对球孢白僵菌产孢的潜在用途。使用30克基质和0.3微升分生孢子悬浮液（1×10⁶个分生孢子/毫升）评估生长和产孢情况。在培养10天后（湿度70±10%，温度（T）=29±1℃），大米（2.00×10⁶个分生孢子/克基质）、角豆树（2.36×10⁶个分生孢子/克）、麦芽A（1.22×10⁶个分生孢子/克）和麦芽B（1.75×10⁶个分生孢子/克）的分生孢子产量最高。所得分生孢子对椰心叶甲的杀虫活性高于80%。这些新的原始基质可能是生产用于生物防治各种害虫的昆虫病原真菌的可行替代品。
