Tiboni O, Di Pasquale G
Biochim Biophys Acta. 1987 Feb 27;908(2):113-22. doi: 10.1016/0167-4781(87)90050-9.
The gene encoding ribosomal proteins S12 and probably S7 as well as protein synthesis elongation factors Tu (EF-Tu) and G (EF-G) of Spirulina platensis have been identified and cloned. Gene expression was determined for ribosomal protein S12 by genetic complementation of the appropriate Escherichia coli mutant, whereas for the EF-Tu gene it was determined by production of the protein in E. coli minicells. On the basis of these experiments we suggest the following gene order in the S. platensis chromosome: S12, S7, EF-G, EF-Tu.
钝顶螺旋藻核糖体蛋白S12以及可能的S7、蛋白质合成延伸因子Tu（EF-Tu）和G（EF-G）的编码基因已被鉴定和克隆。通过对合适的大肠杆菌突变体进行遗传互补来确定核糖体蛋白S12的基因表达，而对于EF-Tu基因，则通过在大肠杆菌微小细胞中产生该蛋白质来确定其基因表达。基于这些实验，我们提出钝顶螺旋藻染色体中的基因顺序如下：S12、S7、EF-G、EF-Tu。
