Faculty of Pharmacy and Biotechnology, Dept. of Pharmaceutical Biology, German Univ. in Cairo, Cairo, Egypt.
Faculty of Pharmacy and Biotechnology, Dept. of Pharmacology and Toxicology, German Univ. in Cairo, Cairo, Egypt.
J Food Sci. 2019 May;84(5):1012-1022. doi: 10.1111/1750-3841.14587. Epub 2019 Apr 24.
Dietary phenolics are known for their potent antioxidant and anti-inflammatory activities, making them promising candidates for protection against neuroinflammation and neurodegeneration. Hydroalcohol extract of Egyptian species of Corchorus olitorius L. (Co) leaves was investigated for its neuroprotective effects in a lipopolysaccharide-induced neuroinflammatory mouse model. Twenty five metabolites were characterized from the bioactive extract using high-performance liquid chromatography HPLC/PDA/HRESI/MS , revealing 1,5-dicaffeoylquinic acid (Co11) as one of the major constituents (5.7%), which was isolated and its identity was confirmed by spectral data as first report. Co significantly protected microglia against H O -induced cytotoxicity and immunohistochemistry showed reduced expression of the astrocytic marker, glial fibrillary acidic protein, and the inflammatory marker, cyclooxygenase-2. These findings correlated with significant improvement of cognitive functions and reduction of LPS-induced neurodegeneration in Co-treated mice as revealed by histopathology. The current study shows promising effects of Co in limiting neurodegeneration and cognitive impairment caused by neuroinflammation and glial cell activation. PRACTICAL APPLICATION: Information presented here shed light on the promising effects of Corchorus olitorius (Co) for the modulation of neuroinflammatory pathways improving the neuroinflammation-related neurodegeneration and cognitive decline. This makes Co a promising candidate as a nutraceutical supplement to be used against neuroinflammation-related disorders.
膳食酚类物质以其强大的抗氧化和抗炎活性而闻名，因此它们是预防神经炎症和神经退行性变的有希望的候选物。研究了埃及藜 Corchorus olitorius L.（Co）叶的水醇提取物对脂多糖诱导的神经炎症小鼠模型的神经保护作用。使用高效液相色谱 HPLC/PDA/HRESI/MS 从生物活性提取物中鉴定出 25 种代谢产物，揭示 1,5-二咖啡酰奎宁酸（Co11）是主要成分之一（5.7%），这是首次分离并通过光谱数据确认其结构。Co 显著保护小胶质细胞免受 H 2 O 2 诱导的细胞毒性，免疫组织化学显示星形胶质细胞标志物胶质纤维酸性蛋白和炎症标志物环氧化酶-2 的表达减少。这些发现与 Co 治疗小鼠认知功能的显著改善和 LPS 诱导的神经退行性变的减少相关，这在组织病理学中得到了证实。本研究显示 Co 在限制神经炎症和小胶质细胞激活引起的神经退行性变和认知障碍方面具有有希望的效果。实际应用：这里介绍的信息表明，Corchorus olitorius（Co）具有调节神经炎症途径的有希望的效果，可改善与神经炎症相关的神经退行性变和认知能力下降。这使得 Co 成为一种有前途的候选物，可以作为一种营养补充剂，用于治疗与神经炎症相关的疾病。
