采用预富集技术从鸡肉中分离李斯特菌的发生率及回收率

Incidence and Recovery of Listeria from Chicken with a Pre-enrichment Technique.

作者信息

Varabioff Y

机构信息

Queensland Food Research Laboratories, Department of Primary Industries, 19 Hercules Street, Hamilton, Q. 4007, Australia.

出版信息

J Food Prot. 1990 Jul;53(7):555-557. doi: 10.4315/0362-028X-53.7.555.

DOI:10.4315/0362-028X-53.7.555

PMID:31018355

Abstract

Eighty frozen chickens from four processors were purchased from retail stores in Brisbane. Forty-eight fresh chicken carcasses and 32 (16 hot and 16 chilled) wash-water samples from each of the four processors were also collected. The isolation of Listeria was achieved by a pre-enrichment procedure which allowed the recovery of injured cells. Listeria monocytogenes was isolated from 12 (15%) of the frozen chickens. Nine (11.2%) isolates were confirmed to be serotype 1 and three (3.8%) serotype 4. Fourteen (17.5%) of the frozen chickens were also contaminated by Listeria innocua . One (2.1%) sample of the fresh chickens yielded L. monocytogenes serotype 1 and five (10.4%) had L. innocua . L. monocytogenes serotype 1 was recovered from two (6.2%) samples of chilled wash water, but no Listeria were detected in hot wash water.

摘要

从布里斯班的零售商店购买了来自四个加工厂的80只冷冻鸡。还从这四个加工厂分别采集了48个新鲜鸡 carcasses 和32个（16个热的和16个冷的）冲洗水样。通过预富集程序实现了李斯特菌的分离，该程序可使受损细胞得以复苏。从12只（15%）冷冻鸡中分离出了单核细胞增生李斯特菌。9株（11.2%）分离株被确认为血清型1，3株（3.8%）为血清型4。14只（17.5%）冷冻鸡还被无害李斯特菌污染。1份（2.1%）新鲜鸡样本检出血清型1的单核细胞增生李斯特菌，5份（10.4%）含有无害李斯特菌。从2份（6.2%）冷冲洗水样中分离出了血清型1的单核细胞增生李斯特菌，但在热冲洗水中未检测到李斯特菌。