Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.
Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.
Sci Total Environ. 2019 Jun 10;668:958-965. doi: 10.1016/j.scitotenv.2019.03.078. Epub 2019 Mar 7.
GY1, a novel microbial consortium with efficient ability to degrade decabromodiphenyl ether (BDE-209) has been isolated and the sequencing analysis has been conducted. The results revealed that Hyphomicrobium, Pseudomonas, Aminobacter, Sphingopyxis, Chryseobacterium, Bacillus, Pseudaminobacter, Stenotrophomonas, Sphingobacterium and Microbacterium were the dominant genera, and the function genes involved in BDE-209 conversion were predicted by PICRUSt. When BDE-209 concentration increased from 0.5 to 10mg/L, its degradation efficiency declined from 57.2% to 22.3%. Various kinds of debrominated metabolites were detected during the biodegradation process, including BDE-208, BDE-207, BDE-206, BDE-205, BDE-190, BDE-181, BDE-155, BDE-154, BDE-99, BDE-47, BDE-17 and BDE-7. Also, the proportion of necrotic cells was observed during GY1 mediated degradation of BDE-209 to reveal the changes of cells viability under BDE-209 stress. Subsequent analysis showed that the reaction of BDE-209 with GY1 was a detoxification process and bioaugmentation with GY1 effectively enhanced BDE-209 degradation in actual water and water-sediment system.
已分离出一种能有效降解十溴联苯醚（BDE-209）的新型微生物共生体 GY1，并进行了测序分析。结果表明，优势属为 Hyphomicrobium、Pseudomonas、Aminobacter、Sphingopyxis、Chryseobacterium、Bacillus、Pseudaminobacter、Stenotrophomonas、Sphingobacterium 和 Microbacterium，并通过 PICRUSt 预测了参与 BDE-209 转化的功能基因。当 BDE-209 浓度从 0.5mg/L 增加到 10mg/L 时，其降解效率从 57.2%下降到 22.3%。在生物降解过程中检测到各种脱溴代谢物，包括 BDE-208、BDE-207、BDE-206、BDE-205、BDE-190、BDE-181、BDE-155、BDE-154、BDE-99、BDE-47、BDE-17 和 BDE-7。此外，在 GY1 介导的 BDE-209 降解过程中观察到坏死细胞的比例，以揭示 BDE-209 胁迫下细胞活力的变化。随后的分析表明，BDE-209 与 GY1 的反应是一种解毒过程，生物强化作用有效地增强了实际水体和水-沉积物系统中 BDE-209 的降解。
