Chemical Engineering Department, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. M. García Barragán # 1451, C.P. 44430 Guadalajara, Jalisco, Mexico.
Electronics Department, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. M. García Barragán #1421, C.P. 44430 Guadalajara, Jalisco, Mexico.
Int J Mol Sci. 2019 Apr 23;20(8):1978. doi: 10.3390/ijms20081978.
Anthropogenic activities have increased the amount of urban wastewater discharged into natural aquatic reservoirs containing a high amount of nutrients such as phosphorus (Pi and PO 4 - 3 ), nitrogen (NH 3 and NO 3 - ) and organic contaminants. Most of the urban wastewater in Mexico do not receive any treatment to remove nutrients. Several studies have reported that an alternative to reduce those contaminants is using consortiums of microalgae and endogenous bacteria. In this research, a genome-scale biochemical reaction network is reconstructed for the co-culture between the microalga and the bacterium . Metabolic Pathway Analysis (MPA), is applied to understand the metabolic capabilities of the co-culture and to elucidate the best conditions in removing nutrients. Theoretical yields for phosphorus removal under photoheterotrophic conditions are calculated, determining their values as 0.042 mmol of PO 4 - 3 per g DW of , 19.43 mmol of phosphorus (Pi) per g DW of and 4.90 mmol of phosphorus (Pi) per g DW of . Similarly, according to the genome-scale biochemical reaction network the theoretical yields for nitrogen removal are 10.3 mmol of NH 3 per g DW of and 7.19 mmol of NO 3 - per g DW of . Thus, this research proves the metabolic capacity of these microorganisms in removing nutrients and their theoretical yields are calculated.
人为活动增加了排入含有大量营养物质（如磷（Pi 和 PO 4 - 3 ）、氮（NH 3 和 NO 3 - ）和有机污染物）的天然水水库中的城市废水的数量。墨西哥的大部分城市废水都没有经过任何处理来去除营养物质。多项研究报告称，减少这些污染物的一种替代方法是使用微藻和内源性细菌的联合体。在这项研究中，为 和 之间的共培养重建了一个基因组规模的生化反应网络。代谢途径分析（MPA）用于了解共培养的代谢能力，并阐明去除营养物质的最佳条件。在光异养条件下计算出磷去除的理论产率，确定其值分别为每克 DW 的 0.042 mmol 的 PO 4 - 3 、每克 DW 的 19.43 mmol 的磷（Pi）和每克 DW 的 4.90 mmol 的磷（Pi）。同样，根据基因组规模的生化反应网络，氮去除的理论产率为每克 DW 的 10.3 mmol 的 NH 3 和每克 DW 的 7.19 mmol 的 NO 3 - 。因此，这项研究证明了这些微生物去除营养物质的代谢能力，并计算了它们的理论产率。
