Allaway David, Gilham Matt, Wagner-Golbs Antje, Maldonado Sandra González, Haydock Richard, Colyer Alison, Stockman Jonathan, Watson Phillip
WALTHAM Centre for Pet Nutrition, Freeby Lane, Waltham-on-the-Wolds, Leicestershire LE14 4RT, UK.
Metanomics Health GmbH, Tegeler Weg 33, 10589 Berlin, Germany.
J Nutr Sci. 2019 Apr 10;8:e13. doi: 10.1017/jns.2019.12. eCollection 2019.
Short-term feeding studies have highlighted a phenomenon in Ca regulation that raises concerns around Ca absorption in dogs that may make an impact on commercial diets near to the maximum recommended level. A recent study to determine responses in dogs fed one of two diets differing in dietary Ca over 40 weeks found no evidence to suggest a concern across a range of biological parameters hypothesised to be affected by Ca. Unforeseen consequences of dietary Ca could have occurred and metabolic profiling was deemed a suitable data-driven approach to identify effects of dietary Ca. The objectives were to compare the fasted plasma metabolome (sampled at 8-week intervals over 40 weeks) of dogs fed one of two diets, near to the minimum and maximum recommended levels of dietary Ca. Comparisons with the control diet were also investigated across the postprandial time course (1-4 h) following acute (1 d) and long-term (24 weeks) feeding of the test diet. Comparing fasted plasma samples at each time point, no significant effect (adjusted < 0·05) of diet on metabolites was observed. In the postprandial state, only phosphate was consistently different between diets and was explained by additional dietary P to maintain Ca:P. Metabolic profiling analysis supports the view that the dietary Ca upper limit is safe. Additionally, the canine plasma metabolome was characterised, providing insights into the stability of individual profiles across 40 weeks, the response to consumption of a nutritionally complete meal over a 4 h postprandial time course and different kinetic categories of postprandial absorption.
短期喂养研究突出了钙调节中的一种现象，这引发了人们对犬类钙吸收的担忧，因为这可能会对接近最大推荐水平的商业饮食产生影响。最近一项旨在确定喂食两种钙含量不同的饮食之一的犬类在40周内的反应的研究发现，没有证据表明在一系列假设受钙影响的生物学参数方面存在问题。饮食钙可能产生了不可预见的后果，代谢谱分析被认为是一种合适的数据驱动方法，用于识别饮食钙的影响。目标是比较喂食两种接近饮食钙最低和最高推荐水平的饮食之一的犬类的空腹血浆代谢组（在40周内每隔8周取样）。还研究了在急性（1天）和长期（24周）喂食测试饮食后的餐后时间进程（1 - 4小时）中与对照饮食的比较。在每个时间点比较空腹血浆样本，未观察到饮食对代谢物有显著影响（调整后P < 0·05）。在餐后状态下，饮食之间只有磷酸盐始终存在差异，这是由额外的饮食磷来维持钙磷比所解释的。代谢谱分析支持饮食钙上限是安全的这一观点。此外，对犬类血浆代谢组进行了表征，深入了解了个体谱在40周内的稳定性、在4小时餐后时间进程中对营养完整餐食摄入的反应以及餐后吸收的不同动力学类别。
