Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, Bejaia, Algérie.
Laboratoire d'Ecologie et d'Environnement, FSNV, Université de Bejaia, Bejaia, Algérie.
Microb Drug Resist. 2019 Sep;25(7):1057-1062. doi: 10.1089/mdr.2018.0471. Epub 2019 Apr 25.
The aim of this study was to screen for the presence of carbapenemase-producing Enterobacteriaceae (CPE) isolates from bat guano in Bejaia, Algeria. Guano samples ( = 110) were collected in Aokas's cave, Bejaia, Algeria, between March and May 2016. Samples were plated on MacConkey agar supplemented with ertapenem (0.5 mg/L) and vancomycin (32 mg/L). The isolates were identified and antimicrobial susceptibility was determined using disk diffusion method. Carbapenemase, extended spectrum β-lactamases, plasmid-mediated AmpC, and plasmid-mediated quinolone resistance genes were studied using PCR and sequencing. Clonal relatedness was studied using multilocus sequence typing (MLST). Two CPE isolates were identified as . PCR and sequencing identified the OXA-48 in one strain (CS34) and KPC-3 in the other strain (CS63). CS63 was found to carry and genes. The MLST showed that CS63 was assigned to ST512, whereas CS34 belonged to ST1878. This is the first description of CPE from bats' guano.
本研究旨在从阿尔及利亚贝贾亚的蝙蝠粪便中筛选产碳青霉烯酶肠杆菌科（CPE）分离株。2016 年 3 月至 5 月期间，在阿尔及利亚贝贾亚的 Aokas 洞穴中采集了 110 份粪便样本。将样本接种于含有厄他培南（0.5mg/L）和万古霉素（32mg/L）的 MacConkey 琼脂上。采用纸片扩散法鉴定分离株并进行药敏试验。采用 PCR 和测序法研究碳青霉烯酶、超广谱β-内酰胺酶、质粒介导的 AmpC 和质粒介导的喹诺酮耐药基因。采用多位点序列分型（MLST）研究克隆相关性。鉴定出两株 CPE 分离株为. PCR 和测序鉴定出一株 （CS34）的 OXA-48 和另一株 （CS63）的 KPC-3。CS63 携带 和 基因。MLST 显示 CS63 被分配到 ST512，而 CS34 属于 ST1878。这是首次从蝙蝠粪便中描述 CPE。
