Mairi Assia, Touati Abdelaziz, Pantel Alix, Yahiaoui Martinez Alex, Ahmim Mourad, Sotto Albert, Dunyach-Remy Catherine, Lavigne Jean-Philippe
Laboratoire d'Ecologie Microbienne, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, Algeria.
Bacterial Virulence and Chronic Infection, Institut National de la Santé et de la Recherche Médicale U1047, University Montpellier, 30908 Nîmes, France.
Microorganisms. 2021 Oct 31;9(11):2264. doi: 10.3390/microorganisms9112264.
Methicillin-resistant (MRSA) is a widespread pathogen that could cause different illnesses in both human and animals. Presence of MRSA in animals raises concerns of their capacity to act as reservoirs, particularly in wild animals. This study aimed to characterize the resistance and virulence patterns of strains isolated from bat guano in Algeria. From March to May 2016, 98 bat guano samples from Aokas's cave (Bejaia, Algeria) were collected. Swabs were taken for microbiological studies. Isolates were identified by Vitek MS system, and antibiotic susceptibility was determined by disk diffusion method. The clonal origin, virulence and antibiotic resistance profiles of isolates were characterized by whole genome sequencing. Eleven strains were obtained from the 98 guano samples. Seven isolates were sensitive to all antibiotics tested and four (36.3%) were resistant to penicillin G, cefoxitin and fusidic acid. The four MRSA isolates were assigned to the sequence type ST149 and related to type 010. These isolates harbored a SCCIV element and the fusidic acid resistance element Q6GD50 (). They carried different virulence genes including several enterotoxins (, enterotoxin locus, , ), and the toxic shock syndrome toxin (). Our results highlight that bat guano may constitute an important reservoir of MRSA strains.
耐甲氧西林金黄色葡萄球菌（MRSA）是一种广泛传播的病原体，可在人类和动物中引发不同疾病。动物体内存在MRSA引发了人们对其作为宿主能力的担忧，尤其是在野生动物中。本研究旨在对从阿尔及利亚蝙蝠粪便中分离出的菌株的耐药性和毒力模式进行特征分析。2016年3月至5月，从奥卡斯洞穴（阿尔及利亚贝贾亚）采集了98份蝙蝠粪便样本。采集拭子用于微生物学研究。通过Vitek MS系统鉴定分离株，并采用纸片扩散法测定抗生素敏感性。通过全基因组测序对分离株的克隆起源、毒力和抗生素耐药谱进行特征分析。从98份粪便样本中获得了11株菌株。7株分离株对所有测试抗生素敏感，4株（36.3%）对青霉素G、头孢西丁和夫西地酸耐药。这4株MRSA分离株被归为序列型ST149，与010型相关。这些分离株含有一个SCCIV元件和夫西地酸耐药元件Q6GD50（）。它们携带不同的毒力基因，包括几种肠毒素（，肠毒素基因座，，）和中毒性休克综合征毒素（）。我们的结果突出表明，蝙蝠粪便可能是MRSA菌株的一个重要宿主。
