College of Environmental Science and Engineering, Guilin University of Technology, Guilin, 541004, China.
College of Environmental Science and Engineering, Guilin University of Technology, Guilin, 541004, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, 541004, China; Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guili, 541004, China.
Environ Pollut. 2019 Jul;250:375-385. doi: 10.1016/j.envpol.2019.04.009. Epub 2019 Apr 14.
Aromatic halogenated chemicals are an unregulated class of byproducts (DBPs) generated from disinfection processes in the water environment. Information on the toxicological interactions, such as antagonism and synergism, present in DBP mixtures remains limited. This study aimed to determine the toxicological effects of aromatic halogenated DBP mixtures on the freshwater bacterium Vibrio qinghaiensis sp.-Q67. The acute toxicities of seven DBPs and their binary mixtures toward V. qinghaiensis sp.-Q67 were determined through microplate toxicity analysis. The toxicities of single DBPs were ranked as follows: 2,5-dibromohydroquinone > 2,4-dibromophenol > 4-bromo-2-chlorophenol ≈ 2,6-dibromo-4-nitrophenol > 2,6-dichloro-4-nitrophenol > 2-bromo-4-chlorophenol > 4-bromophenol. The percentages of synergism (experimental values higher than the predicted concentration addition) on the levels of 50%, 20%, and 10% effective concentrations reached 61%, 41%, and 31%, respectively. These results indicated that the probability of synergism decreased as concentration levels decreased. The synergetic effects of the compounds were dependent on concentration levels and concentration ratios. The proposed quantitative structure-activity relationship model can be used to predict the interactive toxicities exerted by 105 binary DBP mixture rays of 21 DBP mixture systems.
芳香族卤代化学品是一类未经监管的副产物（DBP），它们是在水环境中的消毒过程中产生的。关于 DBP 混合物中存在的毒理学相互作用（如拮抗作用和协同作用）的信息仍然有限。本研究旨在确定芳香族卤代 DBP 混合物对淡水细菌青海弧菌 sp.-Q67 的毒理学影响。通过微孔板毒性分析，确定了七种 DBP 及其二元混合物对 V. qinghaiensis sp.-Q67 的急性毒性。单 DBP 的毒性排序如下：2,5-二溴对苯二酚>2,4-二溴苯酚>4-溴-2-氯苯酚≈2,6-二溴-4-硝基苯酚>2,6-二氯-4-硝基苯酚>2-溴-4-氯苯酚>4-溴苯酚。在 50%、20%和 10%有效浓度水平上，协同作用（实验值高于预测浓度加和）的百分比分别达到 61%、41%和 31%。这些结果表明，协同作用的概率随着浓度水平的降低而降低。化合物的协同作用取决于浓度水平和浓度比。所提出的定量构效关系模型可用于预测 21 个 DBP 混合物系统的 105 个二元 DBP 混合物射线的相互作用毒性。
