College of Environment and Resources, Southwest University of Science and Technology, Mianyang, 621010, China; Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang, 621010, China.
Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang, 621010, China; School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
Ecotoxicol Environ Saf. 2019 Sep 15;179:31-39. doi: 10.1016/j.ecoenv.2019.04.043. Epub 2019 Apr 23.
To analyze the differences between high- and low-accumulation plants in cesium (Cs) uptake and its related mechanism, Brassica juncea (a hyperaccumulation plant for Cs) and Vicia faba (a low-accumulation plant for Cs) were selected as comparative experimental materials. The contributions to Cs uptake of a K-transporter-mediated high-affinity transport system and a K-channel-mediated low-affinity transport system in the two plants were compared and analyzed. The difference between the two plants in the mechanism of Cs uptake was further analyzed using transcription sequence technology. The results show that the transfer characteristics of Cs in the two plants had a similar distribution relationship with K. The contribution rate of the K-channel pathway to Cs uptake was 32.00% in the V. faba seedling roots, which was significantly higher than for B. juncea (9.81%) (P < 0.01); the contribution rate of the K-transporter pathway to Cs uptake of the B. juncea seedlings was 32.08%, which was significantly higher than that of the V. faba seedlings (17.13%)(P < 0.05). Other uptake pathways also mediated the uptake of Cs by roots in B. juncea and V. faba (contribution rate: 54.92-60.09% and 42.18-59.73%, respectively). The transcriptome sequencing results confirmed that Cs-induced treatment significantly inhibited the expression of the K-transporter protein and K-channel protein-related genes in the V. faba roots, but it had no significant effect on the expression of related genes in the B. juncea roots. Thus, one reason for the significant difference between the two plant in the accumulation of Cs is that Cs inhibited the expression of related transporter protein genes in the V. faba roots.
为了分析高积累和低积累植物在铯（Cs）吸收及其相关机制上的差异，选择芥菜（超积累植物）和蚕豆（低积累植物）作为比较实验材料。比较和分析了两种植物中钾转运体介导的高亲和力转运系统和钾通道介导的低亲和力转运系统对 Cs 吸收的贡献。利用转录序列技术进一步分析了两种植物吸收 Cs 机制的差异。结果表明，两种植物中 Cs 的转移特性与 K 具有相似的分布关系。K 通道途径对蚕豆幼苗根系 Cs 吸收的贡献率为 32.00%，明显高于芥菜（9.81%）（P < 0.01）；芥菜幼苗 K 转运体途径对 Cs 吸收的贡献率为 32.08%，明显高于蚕豆幼苗（17.13%）（P < 0.05）。其他吸收途径也介导了 Cs 在芥菜和蚕豆根系中的吸收（贡献率：分别为 54.92-60.09%和 42.18-59.73%）。转录组测序结果证实，Cs 诱导处理显著抑制了蚕豆根系中 K 转运体蛋白和 K 通道蛋白相关基因的表达，但对芥菜根系中相关基因的表达没有显著影响。因此，两种植物在 Cs 积累方面存在显著差异的一个原因是 Cs 抑制了蚕豆根系中相关转运蛋白基因的表达。
