前列腺素从培养的人小梁网细胞和巩膜成纤维细胞中的合成与释放。

Prostaglandin synthesis and release from cultured human trabecular-meshwork cells and scleral fibroblasts.

作者信息

Gerritsen M E, Weinstein B I, Gordon G G, Southren A L

出版信息

Exp Eye Res. 1986 Dec;43(6):1089-102. doi: 10.1016/0014-4835(86)90085-0.

Abstract

Human trabecular-meshwork (HTM) cells in culture convert arachidonic acid to two products: PGE2 and 6-keto PGF1 alpha. Prostaglandin PGE2 was the major product of arachidonic-acid metabolism. The synthesis and release of PGE2 and 6-keto PGF1 alpha was inhibited by a 15-min pre-treatment with indomethacin (5 X 10(-6) M) or a 4-24-hr incubation with 10(-7) M dexamethasone. The effects of dexamethasone could be prevented by cycloheximide (0.5 micrograms ml-1), or actinomycin D (2 micrograms ml-1). Prostaglandin E2 synthesis and release by these HTM cells from two different individuals could be stimulated by bradykinin, arachidonic acid and the calcium ionophore, A23187.

摘要

培养的人小梁网（HTM）细胞将花生四烯酸转化为两种产物：前列腺素E2（PGE2）和6-酮前列腺素F1α。前列腺素PGE2是花生四烯酸代谢的主要产物。用吲哚美辛（5×10⁻⁶ M）预处理15分钟或用10⁻⁷ M地塞米松孵育4 - 24小时可抑制PGE2和6-酮前列腺素F1α的合成与释放。地塞米松的作用可被环己酰亚胺（0.5微克/毫升）或放线菌素D（2微克/毫升）阻断。缓激肽、花生四烯酸和钙离子载体A23187可刺激来自两个不同个体的这些HTM细胞合成和释放前列腺素E2。