Andersen J R, Borggaard B, Olsen E B, Stimpel H, Nyholm H C, Schroeder E
Eur J Obstet Gynecol Reprod Biol. 1986 Nov;23(3-4):159-66. doi: 10.1016/0028-2243(86)90144-9.
Human decidual explants were incubated in vitro in media containing 10(-5) mol/l ouabain or 10(-4) mol/l ouabain or 0.5 X 10(-4) mol/l bumetanide to change the intracellular ionic concentrations. In isosmotic incubations (osmolality 315 mmol/kg), no significant effect of either 10(-5) mol/l ouabain or 0.5 X 10(-4) mol/l bumetanide was found on the decidual prolactin secretion (D-PRL-s). 10(-4) mol/l ouabain significantly decreased both decidual prolactin production and D-PRL-s (P less than 0.05) and was therefore not used for the following cross-over experiments. Hyperosmotic media (387 mmol/kg) were produced by changing the concentration of either sodium chloride, potassium chloride, or mannitol. All increased D-PRL-s compared with the isosmotic media (315 mmol/kg). 10(-5) mol/l ouabain significantly diminished the increase otherwise elicited by the sodium chloride and the mannitol hyperosmotic media. However, in the hyperosmotic potassium chloride medium with 10(-5) mol/l ouabain, the D-PRL-s remained increased. The hyperosmotic medium (252 mmol/kg) reduced D-PRL-s compared with the isosmotic media (315 mmol/kg) and no significant effect of ouabain was found. Bumetanide did not change the D-PRL-s into any of the hypo- or hyperosmotic media compared with the secretion at 315 mmol/kg. Based on experience from other cell types, the results further indicate that the intracellular ionic concentrations could be of importance to the secretion of decidual prolactin in vitro.
将人蜕膜外植体在含有10⁻⁵mol/L哇巴因、10⁻⁴mol/L哇巴因或0.5×10⁻⁴mol/L布美他尼的培养基中进行体外培养，以改变细胞内离子浓度。在等渗培养（渗透压315mmol/kg）中，发现10⁻⁵mol/L哇巴因或0.5×10⁻⁴mol/L布美他尼对蜕膜催乳素分泌（D-PRL-s）均无显著影响。10⁻⁴mol/L哇巴因显著降低了蜕膜催乳素的产生和D-PRL-s（P<0.05），因此未用于后续的交叉实验。通过改变氯化钠、氯化钾或甘露醇的浓度制备高渗培养基（387mmol/kg）。与等渗培养基（315mmol/kg）相比，所有高渗培养基均增加了D-PRL-s。10⁻⁵mol/L哇巴因显著减弱了氯化钠和甘露醇高渗培养基原本引起的增加。然而，在含有10⁻⁵mol/L哇巴因的高渗氯化钾培养基中，D-PRL-s仍然增加。与等渗培养基（315mmol/kg）相比，低渗培养基（252mmol/kg）降低了D-PRL-s，且未发现哇巴因有显著影响。与315mmol/kg时的分泌相比，布美他尼在任何低渗或高渗培养基中均未改变D-PRL-s。基于其他细胞类型的经验，结果进一步表明细胞内离子浓度可能对体外蜕膜催乳素的分泌很重要。
