Andersen J R, Borggaard B, Ornvold K, Knoth M
Acta Endocrinol (Copenh). 1982 Aug;100(4):623-9. doi: 10.1530/acta.0.1000623.
Using a paired design as well as a cross-over design both with 16 h incubation periods, we have incubated human decidua in hyposmotic, isosmotic, and sodium chloride hyperosmotic media. In the cross-over design each tissue aliquot was subjected to 3 media with different osmolalities, one of which was isosmotic. Thus, intensive utilization of the tissue was possible. The results of the cross-over study were identical with the result of the paired design. We found a decreased secretion of prolactin in hyposmotic environments, whereas hyperosmotic environments up to an osmolality about 400 mmol/kg gave a dose related increase in the prolactin secretion into the medium as well as in the total content of prolactin in the tissue and the medium. Excessive hyperosmotic medium decreased the secretion. The enhanced secretion was an effect of increased production, which could be blocked by cycloheximide. We found no feedback of prolactin on its own secretion.
采用配对设计以及交叉设计，两种设计的孵育期均为16小时，我们将人蜕膜置于低渗、等渗和氯化钠高渗培养基中进行孵育。在交叉设计中，每个组织等分试样都要置于3种不同渗透压的培养基中，其中一种是等渗的。因此，可以充分利用组织。交叉研究的结果与配对设计的结果相同。我们发现，在低渗环境中催乳素分泌减少，而高达约400 mmol/kg渗透压的高渗环境会使培养基中催乳素分泌以及组织和培养基中催乳素总含量呈剂量相关增加。过高渗透压的培养基会使分泌减少。分泌增强是产量增加的结果，这可被环己酰亚胺阻断。我们未发现催乳素对其自身分泌有反馈作用。
