使用¹²⁵I-蛋白A放射免疫分析法对D、Du和d红细胞上的Rh1（Rho，D）抗原进行定量比较。

Quantitative comparison of Rh1 (Rho, D) antigen on D, Du and d red cells by radioimmunoassay using 125I-protein A.

作者信息

Fukushima H, Matsuba K, Ota M, Segawa M, Yonemura I, Hasekura H

出版信息

Int J Biochem. 1986;18(12):1147-9. doi: 10.1016/0020-711x(86)90090-x.

Abstract

Relative Rh1 (Rho, D) antigen contents of the red Rh: 1 (Rh positive, D), Rh: wl (Rh variant, Du) and Rh: -1 (Rh negative, d) cells were estimated from the quantity of 125I-protein A bound to the sensitized red cells. The isotope binding activity to both D and Du cells decreased in parallel with the dilution of anti-D serum. The relative amount of the 125I-protein A bound to Du cells was about one-sixth that of D cells without papain treatment, while no isotope binding was observed in d cells. The Du red cells were quantitatively deficient in Rh1 (Rho, D) antigen activity compared with the D cells. A radioimmunoassay using 125I-protein A was a very useful method for studies regarding measuring the relative amounts of various blood group antigens.

摘要

通过与致敏红细胞结合的¹²⁵I-蛋白A的量，估算Rh: 1（Rh阳性，D）、Rh: wl（Rh变异型，Du）和Rh: -1（Rh阴性，d）红细胞的相对Rh1（Rho，D）抗原含量。与抗-D血清的稀释度平行，¹²⁵I-蛋白A与D细胞和Du细胞的结合活性均降低。未经木瓜蛋白酶处理时，与Du细胞结合的¹²⁵I-蛋白A的相对量约为D细胞的六分之一，而在d细胞中未观察到同位素结合。与D细胞相比，Du红细胞的Rh1（Rho，D）抗原活性在数量上不足。使用¹²⁵I-蛋白A的放射免疫测定法是研究测量各种血型抗原相对含量的非常有用的方法。