人类Rh血型的Rh1(D)、2(C)、3(E)、4(c)、5(e)和25(LW)抗原在胎儿红细胞膜中的定位

Localization of Rh1(D), 2(C), 3(E), 4(c), 5(e) and 25(LW) antigens of human Rh blood groups in fetal erythrocyte membranes.

作者信息

Fukushima H, Segawa M, Ota M, Yonemura I, Hiraide K, Hasekura H

出版信息

Comp Biochem Physiol B. 1987;86(2):303-5. doi: 10.1016/0305-0491(87)90295-1.

Abstract

The fetal erythrocyte membranes were partially solubilized with Triton X-100 at the low concentration (0.5%). The localizations of Rh1(D), 2(C), 3(E), 4(c), 5(e) and 25(LW) were investigated. Using hemagglutination inhibition assay, Rh1(D) antigen activity was observed in the Triton-treated membrane (Triton shell) containing mainly band 1, 2 (spectrin), band 5 (actin), band 4.1 and a part of band 3, while Rh2(C), 3(E), 4(c), 5(e) and 25(LW) antigens were detected in the supernatant containing band 3, 6, 2.2, 2.3 and 4.2. It is suggested that: Rh1(D) antigen would associate with cytoskeleton matrix of fetal erythrocyte membranes; Rh1(D) and Rh25(LW) antigens might be integral membrane proteins, while Rh2(C), 3(E), 4(c) and 5(e) antigens would be surface membrane proteins which are easily released from membranes by EDTA, mercaptoethanol and alkaline treatments.

摘要

将胎儿红细胞膜用低浓度（0.5%）的曲拉通X-100进行部分溶解。研究了Rh1（D）、2（C）、3（E）、4（c）、5（e）和25（LW）的定位。采用血凝抑制试验，在主要含有带1、2（血影蛋白）、带5（肌动蛋白）、带4.1和部分带3的经曲拉通处理的膜（曲拉通壳）中观察到Rh1（D）抗原活性，而在含有带3、6、2.2、2.3和4.2的上清液中检测到Rh2（C）、3（E）、4（c）、5（e）和25（LW）抗原。提示：Rh1（D）抗原可能与胎儿红细胞膜的细胞骨架基质相关联；Rh1（D）和Rh25（LW）抗原可能是整合膜蛋白，而Rh2（C）、3（E）、4（c）和5（e）抗原可能是表面膜蛋白，它们很容易通过EDTA、巯基乙醇和碱性处理从膜上释放出来。