在针叶樱桃果实中，Smirnoff-Wheeler途径中多种酶的高表达水平对于抗坏血酸的高积累很重要。
High levels of expression of multiple enzymes in the Smirnoff-Wheeler pathway are important for high accumulation of ascorbic acid in acerola fruits.
作者信息
Suekawa Marina, Fujikawa Yukichi, Inoue Akari, Kondo Takayuki, Uchida Eriko, Koizumi Takeshi, Esaka Muneharu
机构信息
a Graduate School of Biosphere Sciences, Hiroshima University , Higashi-Hiroshima , Japan.
b Department of Bioscience and Bioinformatics, Kyushu Institute of Technology , Iizuka , Japan.
出版信息
Biosci Biotechnol Biochem. 2019 Sep;83(9):1713-1716. doi: 10.1080/09168451.2019.1608808. Epub 2019 Apr 25.
Acerola fruits contain abundant ascorbic acid (AsA). The gene expression levels of three upstream enzymes in the primary AsA biosynthesis pathway were correlated with AsA contents in the fruits of two acerola cultivars. Multiple overexpression of the enzymes increased AsA contents, suggesting their high expression is important for high AsA accumulation in acerola fruits and the breeding of AsA-rich plants. AsA: ascorbic acid; PMI: phosphomannose isomerase; PMM: phosphomannomutase; GMP: GDP-d-mannose pyrophosphorylase; GME: GDP-d-mannose 3',5'-epimerase; GGP: GDP-l-galactose phosphorylase; GPP: l-galactose-1-phosphate phosphatase; GDH: l-galactose dehydrogenase; GLDH: l-galactono-1,4-lactone dehydrogenase.
针叶樱桃果实含有丰富的抗坏血酸（AsA）。在两个针叶樱桃品种的果实中，初级AsA生物合成途径中三种上游酶的基因表达水平与AsA含量相关。这些酶的多重过表达增加了AsA含量，表明它们的高表达对于针叶樱桃果实中高AsA积累以及富含AsA植物的育种很重要。AsA：抗坏血酸；PMI：磷酸甘露糖异构酶；PMM：磷酸甘露糖变位酶；GMP：GDP-D-甘露糖焦磷酸化酶；GME：GDP-D-甘露糖3'，5'-差向异构酶；GGP：GDP-L-半乳糖磷酸化酶；GPP：L-半乳糖-1-磷酸磷酸酶；GDH：L-半乳糖脱氢酶；GLDH：L-半乳糖酸-1,4-内酯脱氢酶。
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