Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing, China.
Beijing University of Agriculture, Beijing, China.
PLoS One. 2024 Aug 22;19(8):e0307527. doi: 10.1371/journal.pone.0307527. eCollection 2024.
Ascorbic acid (AsA) is an essential nutritional component and powerful antioxidant in vegetables, and in plants, AsA levels are regulated by light. AsA levels in the leaves of Chinese chive (Allium tuberosum Rottler ex Spr), a popular vegetable, are poorly understood. Thus, this study was performed to assess the influence of light on AsA biosynthesis in chive and select related genes (AtuGGP1 and AtuGME1); in addition, bioinformatic analyses and gene expression level assays were performed. The biological information obtained for AtuGGP1 and AtuGME1 was analysed with several tools, including NCBI, DNAMAN, and MEGA11. After different light treatments were performed, the Chive AsA content and AtuGGP1 and AtuGME1 expression levels were determined. These results suggest that 1) compared with natural light, continuous darkness inhibited AsA synthesis in chives. 2) The amino acid sequences of AtuGGP1 and AtuGME1 are very similar to those of other plants. 3) The trends observed for the expression levels of AtuGGP1 and AtuGME1 were consistent with the AsA content observed in chives. Hence, we speculated that light controls AsA biosynthesis in chives by regulating AtuGGP1 and AtuGME1 expression. This study provided impactful and informative evidence regarding the functions of GGP and GME in chives.
抗坏血酸（AsA）是蔬菜中必需的营养成分和强大的抗氧化剂，在植物中，AsA 水平受光照调节。韭菜（Allium tuberosum Rottler ex Spr）是一种受欢迎的蔬菜，其叶片中的 AsA 水平尚未得到充分了解。因此，本研究旨在评估光照对韭菜中 AsA 生物合成的影响，并筛选相关基因（AtuGGP1 和 AtuGME1）；此外，还进行了生物信息学分析和基因表达水平测定。利用 NCBI、DNAMAN 和 MEGA11 等多种工具对 AtuGGP1 和 AtuGME1 的生物信息进行了分析。在进行不同的光照处理后，测定了韭菜 AsA 含量和 AtuGGP1 和 AtuGME1 的表达水平。这些结果表明：1）与自然光相比，连续黑暗抑制了韭菜中 AsA 的合成。2）AtuGGP1 和 AtuGME1 的氨基酸序列与其他植物非常相似。3）AtuGGP1 和 AtuGME1 的表达水平与韭菜中观察到的 AsA 含量趋势一致。因此，我们推测光照通过调节 AtuGGP1 和 AtuGME1 的表达来控制韭菜中 AsA 的生物合成。本研究为 GGP 和 GME 在韭菜中的功能提供了有影响力和信息丰富的证据。
