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家蚕肽通过TLR2诱导的MyD88依赖途径诱导M1型极化和Th1激活的作用。

Effect of silkworm peptide on inducting M1 type polarization and Th1 activation via TLR2-induced MyD88-dependent pathway.

作者信息

Zhu Guanglai, Gui Zhongzheng

机构信息

School of Biotechnology Jiangsu University of Science and Technology Zhenjiang China.

Department of Aquatic Science and Technology Jiangsu Animal Husbandry and Veterinary College Taizhou China.

出版信息

Food Sci Nutr. 2019 Mar 12;7(4):1251-1260. doi: 10.1002/fsn3.954. eCollection 2019 Apr.

DOI:10.1002/fsn3.954
PMID:31024698
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6475741/
Abstract

The aim of this study was to explore immune activity and molecular mechanism of silkworm peptide. The cell subsets induced by silkworm peptides were detected by flow cytometry. The IFN-γ and IL-4 levels in CD4 cells were measured by ELISA. TLR2 mRNA expression in mouse CD4 T cells was detected by qRT-PCR. Western blot was used to detect the protein expression levels of MyD88 and p-IκB. The growth rate of Lewis lung cancer xenografts in mice of the medium-dose group was significantly reduced, and the tumor volume was significantly smaller than that of the control group on the 14th day. The relative vitality values of spleen lymphocytes in the medium-dose and high-dose groups were higher than the control group. The IFN-γ levels in the medium-dose and high-dose groups were significantly higher than the control group. The levels of IL-4 were no significant change among different groups. Compared with the control group, different doses of silkworm peptide groups could increase the levels of NO, IL-6, IL-12, and IL-1β. Compared with the control group, the protein expression levels of MyD88 and p-IκB in 10 μg/ml group and 20 μg/ml groups were significantly increased compared with the control group. Silkworm peptide could induce Th1 activation and M1 type polarization, which was dose-dependent and was relative to the effect of silkworm peptide on inhibiting tumor growth. Silkworm peptide could directly induce M1 type polarization and Th1 activation via TLR2-induced MyD88-dependent pathway in vitro.

摘要

本研究旨在探讨家蚕肽的免疫活性及分子机制。采用流式细胞术检测家蚕肽诱导的细胞亚群。通过ELISA检测CD4细胞中IFN-γ和IL-4水平。利用qRT-PCR检测小鼠CD4 T细胞中TLR2 mRNA表达。采用Western blot检测MyD88和p-IκB的蛋白表达水平。中剂量组小鼠Lewis肺癌异种移植瘤的生长速率显著降低，在第14天时肿瘤体积显著小于对照组。中剂量组和高剂量组脾淋巴细胞的相对活力值高于对照组。中剂量组和高剂量组的IFN-γ水平显著高于对照组。不同组间IL-4水平无显著变化。与对照组相比，不同剂量的家蚕肽组可提高NO、IL-6、IL-12和IL-1β水平。与对照组相比，10μg/ml组和20μg/ml组的MyD88和p-IκB蛋白表达水平显著升高。家蚕肽可诱导Th1活化和M1型极化，呈剂量依赖性，且与家蚕肽抑制肿瘤生长的作用相关。家蚕肽在体外可通过TLR2诱导的MyD88依赖性途径直接诱导M1型极化和Th1活化。

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