基于基因表达综合数据库鉴定翼状胬肉的致病基因
Identification of pathogenic genes of pterygium based on the Gene Expression Omnibus database.
作者信息
Yue Xiao-Li, Gao Zi-Qing
机构信息
Department of Ophthalmology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, Anhui Province, China.
出版信息
Int J Ophthalmol. 2019 Apr 18;12(4):529-535. doi: 10.18240/ijo.2019.04.01. eCollection 2019.
AIM
To identify the pathogenic genes in pterygium.
METHODS
We obtained mRNA expression profiles from the Gene Expression Omnibus database (GEO) to identify differentially expressed genes (DEGs) between pterygium tissues and normal conjunctiva tissues. The Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway analysis, protein-protein interaction (PPI) network and transcription factors (TFs)-target gene regulatory network was performed to understand the function of DEGs. The expression of selected DEGs were validated by the quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS
A total of 557 DEGs were identified between pterygium and normal individual. In PPI network, several genes were with high degrees such as and and were the most downstream genes regulated by TFs. qRT-PCR results showed that and were significantly up-regulated in pterygium and and were also up-regulated with no significance, which was consistent with the our integrated analysis.
CONCLUSION
The deregulated genes might be involved in the pathology of pterygium and could be used as treatment targets for pterygium.
目的
鉴定翼状胬肉中的致病基因。
方法
我们从基因表达综合数据库（GEO）获取mRNA表达谱，以鉴定翼状胬肉组织与正常结膜组织之间的差异表达基因（DEG）。进行基因本体论、京都基因与基因组百科全书通路分析、蛋白质-蛋白质相互作用（PPI）网络和转录因子（TF）-靶基因调控网络，以了解DEG的功能。通过定量实时聚合酶链反应（qRT-PCR）验证所选DEG的表达。
结果
在翼状胬肉与正常个体之间共鉴定出557个DEG。在PPI网络中，几个基因具有较高的度数，如 和 ， 是受TF调控的最下游基因。qRT-PCR结果显示， 和 在翼状胬肉中显著上调， 和 也上调但无显著性，这与我们的综合分析一致。
结论
失调的基因可能参与翼状胬肉的病理过程，并可作为翼状胬肉的治疗靶点。
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