State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China.
Pest Manag Sci. 2019 Dec;75(12):3312-3322. doi: 10.1002/ps.5454. Epub 2019 Jun 3.
Fusarium head blight (FHB) is a devastating disease of cereal crops worldwide mainly caused by Fusarium graminearum. Due to the unavailability of FHB-resistant wheat cultivars, chemical fungicide application is currently the most effective approach for controlling FHB now. In the last few years, a novel cyanoacrylate fungicide, phenamacril, has been widely used in China for FHB disease management. In previous studies, we identified that myosin I (FgMyo1) is the target of phenamacril and is essential for mycotoxin deoxynivalenol (DON) biosynthesis and fungal growth. However, the regulation of FgMYO1 gene expression is still largely unknown.
In this study, we identified a b-ZIP transcription factor, FgTfmI, which regulates the mRNA expression of FgMYO1 upon phenamacril treatment. The FgTfmI directly binds to the promoter region of FgMYO1, and is required for the upregulation of FgMYO1 in response to phenamacril treatment. The deletion mutant of FgTFMI (ΔFgTfmI) displayed a slight growth defect, while it showed hypersensitivity to phenamacril, but not to other tested fungicides. FgTfmI also contributed to DON biosynthesis and the infection process in planta.
The transcription factor FgTfmI plays an important role in regulating transcription of the genes involved in phenamacril tolerance, DON biosynthesis and virulence in F. graminearum. © 2019 Society of Chemical Industry.
镰刀菌穗腐病（FHB）是一种全球性的破坏性谷物病害，主要由禾谷镰刀菌引起。由于缺乏对 FHB 的小麦品种，化学杀菌剂的应用是目前控制 FHB 的最有效方法。在过去的几年中，一种新型的氰基丙烯酸酯杀菌剂苯霜灵已在中国广泛用于 FHB 病害管理。在之前的研究中，我们鉴定出肌球蛋白 I（FgMyo1）是苯霜灵的靶标，并且是真菌毒素脱氧雪腐镰刀菌烯醇（DON）生物合成和真菌生长所必需的。然而，FgMYO1 基因表达的调控在很大程度上仍然未知。
在这项研究中，我们鉴定了一个 b-ZIP 转录因子 FgTfmI，它在苯霜灵处理时调节 FgMYO1 的 mRNA 表达。FgTfmI 直接结合到 FgMYO1 的启动子区域，并且是响应苯霜灵处理而上调 FgMYO1 所必需的。FgTFMI 的缺失突变体（ΔFgTfmI）表现出轻微的生长缺陷，而对苯霜灵表现出超敏反应，但对其他测试的杀菌剂没有反应。FgTfmI 还参与 DON 生物合成和体内感染过程。
转录因子 FgTfmI 在调节与苯霜灵耐受性、DON 生物合成和禾谷镰刀菌毒力相关的基因转录中起重要作用。© 2019 化学工业协会。
